PDF(Pubmed)
Abstract：
UNASSIGNED: To investigate the structure, composition, and functions of the gut microbiota in elderly patients with hyperlipidemia.
UNASSIGNED: Sixteen older patients diagnosed with hyperlipidemia (M group) and 10 healthy, age-matched normal volunteers (N group) were included. These groups were further subdivided by sex into the male normal (NM, n = 5), female normal (NF, n = 5), male hyperlipidemia (MM, n = 8), and female hyperlipidemia (MF, n = 8) subgroups. Stool samples were collected for high-throughput sequencing of 16S rRNA genes. Blood samples were collected for clinical biochemical index testing.
UNASSIGNED: Alpha- and beta-diversity analyses revealed that the structure and composition of the gut microbiota were significantly different between the M and N groups. The relative abundances of Bacteroides, Parabacteroides, Blautia, Peptococcus, and Bifidobacterium were significantly decreased, while those of Lactobacillus, Helicobacter, and Desulfovibrio were significantly higher in the M group. There were also significant sex-related differences in microbial structure between the NM and NF groups, and between the MM and MF groups. Through functional prediction with PICRUSt 2, we observed distinct between-group variations in metabolic pathways associated with the gut microbiota and their impact on the functionality of the nervous system. Pearson\'s correlation coefficient was used as a distance metric to build co-abundance networks. A hypergeometric test was used to detect taxonomies with significant enrichment in specific clusters. We speculated that modules with Muribaculaceae and Lachnospiraceae as the core microbes play an important ecological role in the intestinal microbiota of the M group. The relative intestinal abundances of Agathobacter and Faecalibacterium in the M group were positively correlated with serum triglyceride and low-density lipoprotein levels, while the relative abundance of Bifidobacterium was negatively correlated with the serum lipoprotein a level.
UNASSIGNED: Sixteen older patients diagnosed with hyperlipidemia (M group) and 10 healthy, age-matched normal volunteers (N group) were included. These groups were further subdivided by sex into the male normal (NM, n = 5), female normal (NF, n = 5), male hyperlipidemia (MM, n = 8), and female hyperlipidemia (MF, n = 8) subgroups. Stool samples were collected for high-throughput sequencing of 16S rRNA genes. Blood samples were collected for clinical biochemical index testing.
UNASSIGNED: Alpha- and beta-diversity analyses revealed that the structure and composition of the gut microbiota were significantly different between the M and N groups. The relative abundances of Bacteroides, Parabacteroides, Blautia, Peptococcus, and Bifidobacterium were significantly decreased, while those of Lactobacillus, Helicobacter, and Desulfovibrio were significantly higher in the M group. There were also significant sex-related differences in microbial structure between the NM and NF groups, and between the MM and MF groups. Through functional prediction with PICRUSt 2, we observed distinct between-group variations in metabolic pathways associated with the gut microbiota and their impact on the functionality of the nervous system. Pearson\'s correlation coefficient was used as a distance metric to build co-abundance networks. A hypergeometric test was used to detect taxonomies with significant enrichment in specific clusters. We speculated that modules with Muribaculaceae and Lachnospiraceae as the core microbes play an important ecological role in the intestinal microbiota of the M group. The relative intestinal abundances of Agathobacter and Faecalibacterium in the M group were positively correlated with serum triglyceride and low-density lipoprotein levels, while the relative abundance of Bifidobacterium was negatively correlated with the serum lipoprotein a level.
摘要：
■要研究结构，composition,和老年高脂血症患者肠道菌群的功能。
■16名诊断为高脂血症的老年患者（M组）和10名健康者，纳入年龄匹配的正常志愿者(N组).这些群体按性别进一步细分为男性正常人(NM，n=5),女性正常（NF，n=5),男性高脂血症（MM，n=8),和女性高脂血症（MF，n=8)子组。收集粪便样品用于16SrRNA基因的高通量测序。采集血样进行临床生化指标检测。
■α-和β-多样性分析显示，M组和N组之间的肠道微生物群的结构和组成显着不同。拟杆菌的相对丰度，副杆菌属,Blautia,肽球菌,双歧杆菌明显减少，而乳酸菌，螺杆菌,在M组中，脱硫弧菌和脱硫弧菌明显高于M组。NM和NF组之间的微生物结构也存在显着的性别相关差异，在MM和MF组之间。通过PICRUSt2的功能预测，我们观察到与肠道微生物群相关的代谢途径的不同组间差异及其对神经系统功能的影响。采用皮尔逊相关系数作为距离度量来构建共富网络。使用超几何检验来检测在特定簇中显着富集的分类。我们推测，以Muribaculaceae和Lachnospiaceae为核心微生物的模块在M组的肠道菌群中起着重要的生态作用。M组的肠杆菌和粪杆菌的相对丰度与血清甘油三酯和低密度脂蛋白水平呈正相关,而双歧杆菌的相对丰度与血清脂蛋白a水平呈负相关。
■16名诊断为高脂血症的老年患者（M组）和10名健康者，纳入年龄匹配的正常志愿者(N组).这些群体按性别进一步细分为男性正常人(NM，n=5),女性正常（NF，n=5),男性高脂血症（MM，n=8),和女性高脂血症（MF，n=8)子组。收集粪便样品用于16SrRNA基因的高通量测序。采集血样进行临床生化指标检测。
■α-和β-多样性分析显示，M组和N组之间的肠道微生物群的结构和组成显着不同。拟杆菌的相对丰度，副杆菌属,Blautia,肽球菌,双歧杆菌明显减少，而乳酸菌，螺杆菌,在M组中，脱硫弧菌和脱硫弧菌明显高于M组。NM和NF组之间的微生物结构也存在显着的性别相关差异，在MM和MF组之间。通过PICRUSt2的功能预测，我们观察到与肠道微生物群相关的代谢途径的不同组间差异及其对神经系统功能的影响。采用皮尔逊相关系数作为距离度量来构建共富网络。使用超几何检验来检测在特定簇中显着富集的分类。我们推测，以Muribaculaceae和Lachnospiaceae为核心微生物的模块在M组的肠道菌群中起着重要的生态作用。M组的肠杆菌和粪杆菌的相对丰度与血清甘油三酯和低密度脂蛋白水平呈正相关,而双歧杆菌的相对丰度与血清脂蛋白a水平呈负相关。
