PDF(Pubmed)
Abstract：
The gene family of thaumatin-like proteins (TLPs) plays a crucial role in the adaptation of organisms to environmental stresses. In recent years, fungal secreted proteins (SP) with inducing disease resistance activity in plants have emerged as important elicitors in the control of fungal diseases. Identifying SPs with inducing disease resistance activity and studying their mechanisms are crucial for controlling sheath blight. In the present study, 10 proteins containing the thaumatin-like domain were identified in strain AG4-JY of Rhizoctonia solani and eight of the 10 proteins had signal peptides. Analysis of the TLP genes of the 10 different anastomosis groups (AGs) showed that the evolutionary relationship of the TLP gene was consistent with that between different AGs of R. solani. Furthermore, it was found that RsTLP3, RsTLP9 and RsTLP10 were regarded as secreted proteins for their signaling peptides exhibited secretory activity. Prokaryotic expression and enzyme activity analysis revealed that the three secreted proteins possess glycoside hydrolase activity, suggesting they belong to the TLP family. Additionally, spraying the crude enzyme solution of the three TLP proteins could enhance maize resistance to sheath blight. Further analysis showed that genes associated with the salicylic acid and ethylene pathways were up-regulated following RsTLP3 application. The results indicated that RsTLP3 had a good application prospect in biological control.
摘要：
Thaumatin样蛋白（TLPs）的基因家族在生物体对环境压力的适应中起着至关重要的作用。近年来,在植物中具有诱导抗病活性的真菌分泌蛋白（SP）已成为控制真菌疾病的重要激发子。识别具有诱导抗病活性的SP并研究其机制对于控制纹枯病至关重要。在本研究中,在枯萎病丝核菌的AG4-JY菌株中鉴定出10种含有thaumatin样结构域的蛋白质，10种蛋白质中的8种具有信号肽。对10个不同吻合组（AG）的TLP基因的分析表明，TLP基因的进化关系与solaniR.solani不同AG之间的进化关系一致。此外，发现RsTLP3，RsTLP9和RsTLP10被认为是分泌蛋白，因为它们的信号肽表现出分泌活性。原核表达和酶活性分析表明，这三种分泌蛋白具有糖苷水解酶活性，暗示他们属于TLP家族.此外,喷施三种TLP蛋白的粗酶溶液可以增强玉米对纹枯病的抗性。进一步的分析表明，与水杨酸和乙烯途径相关的基因在RsTLP3应用后上调。结果表明RsTLP3在生物防治中具有良好的应用前景。
