Abstract：
This study aims to explore the correlation between intestinal toxicity and composition changes of Euphorbia ebracteolata before and after Terminalia chebula soup(TCS) processing. Intragastric administration was performed on the whole animal model. By using fecal water content, inflammatory causes, and pathological damage of different parts of the intestinal tract of mice as indexes, the differences in intestinal toxicity of dichloromethane extraction of raw E. ebracteolata(REDE), dichloromethane extraction of TCS, and dichloromethane extraction of E. ebracteolata after simulated TCS processing(STREDE) were compared, so as to investigate the effect of TCS processing on the intestinal toxicity of E. ebracteolata. At the same time, the component databases of E. ebracteolata and T. chebula were constructed, and the composition changes of diterpenoids, tannins, and phenolic acids in the three extracted parts were analyzed by HPLC-TOF-MS. HPLC was used to compare the content of four diterpenoids including ent-11α-hydroxyabicta-8(14), 13(15)-dien-16, 12-olide(HAO), jolkinolide B(JNB), fischeria A(FA), and jolkinolide E(JNE) in the E. ebracteolata before and after processing and the residue of container wall after processing, so as to investigate the effect of TCS processing on the content and structure of the diterpenoids. The results showed that the REDE group could significantly increase the fecal water content and the release levels of TNF-α and IL-1β from each intestinal segment, and intestinal tissue damage was accompanied by significant infiltration of inflammatory cells. However, compared with the REDE group, the intestinal tissue damage in the STREDE group was alleviated, and the infiltration of inflammatory cells decreased. The intestinal toxicity significantly decreased. Mass spectrometry analysis showed that there was no significant difference in the content of diterpenoids of REDE before and after simulated TCS processing, but a large number of tannins and phenolic acids were added. The results of HPLC showed that the content of four diterpenoids of E. ebracteo-lata decreased to varying degrees after TCS processing, ranging from-0.35% to-19.74%, and the decreased part mainly remained in the container wall, indicating that the structure of toxic diterpenoids of E. ebracteolata was not changed after TCS processing. The antagonistic effect of tannic and phenolic acids in the TCS may be the main reason for the reduced intestinal toxicity of E. ebracteolata after TCS processing. The TCS processing for E. ebracteolata is scientific.
摘要：
本研究旨在探讨一品红乳香汤(TCS)炮制前后肠道毒性与成分变化的相关性。在整个动物模型上进行胃内给药。通过使用粪便含水量，炎症原因，以小鼠肠道不同部位的病理损伤为指标,二氯甲烷提取原生E.ebracteolata（REDE）的肠道毒性差异，二氯甲烷萃取TCS，与模拟TCS处理（STREDE）后的二氯甲烷萃取物进行了比较，从而探讨TCS处理对肠毒性的影响。同时,建立了E.ebracteolata和T.chebula的组件数据库，和二萜的成分变化，单宁,用HPLC-TOF-MS分析三个提取部位中的酚酸。HPLC用于比较包括ent-11α-羟基abicta-8（14）在内的四种二萜的含量，13（15）-dien-16，12-内酯（HAO），jolkinolideB(JNB),FischeriaA(FA)，加工前后的E.和jolkinolideE（JNE）以及加工后的容器壁残留物，从而探讨TCS加工对二萜的含量和结构的影响。结果表明,REDE组能显著增加粪便含水量和各肠段TNF-α、IL-1β的释放水平,肠组织损伤伴有明显的炎性细胞浸润。然而,与REDE组相比，STREDE组肠组织损伤得到缓解，炎症细胞浸润减少。肠毒性显著下降。质谱分析表明,模拟TCS处理前后REDE中二萜的含量无显著差异,但是添加了大量的单宁和酚酸。HPLC结果显示,经TCS处理后,4种二萜的含量均有不同程度的降低,范围从-0.35%到-19.74%，减少的部分主要留在集装箱壁，表明TCS处理后，E.ebracteolata的有毒二萜的结构没有改变。单宁酸和酚酸在TCS中的拮抗作用可能是TCS加工后肠毒性降低的主要原因。E.ebracteolata的TCS处理是科学的。
