METHODS: The effects of DC-5163 on breast cancer cells were investigated in vitro and in vivo. Seahorse, glucose uptake, lactate production, and cellular ATP content assays were performed to examine the impact of DC-5163 on cellular glycolysis. Cell viability, colony-forming ability, cell cycle, and apoptosis were assessed by CCK8 assay, colony formation assay, flow cytometry, and immunoblotting respectively. The anticancer activity of DC-5163 in vivo was evaluated in a mouse breast cancer xenograft model.
RESULTS: DC-5163 suppressed aerobic glycolysis and reduced energy supply of breast cancer cells, thereby inhibiting breast cancer cell growth, inducing cell cycle arrest in the G0/G1 phase, and increasing apoptosis. The therapeutic efficacy was assessed using a breast cancer xenograft mouse model. DC-5163 treatment markedly suppressed tumor growth in vivo without inducing evident systemic toxicity. Micro-PET/CT scans revealed a notable reduction in tumor 18F-FDG and 18F-FLT uptake in the DC-5163 treatment group compared to the DMSO control group.
CONCLUSIONS: Our results suggest that DC-5163 is a promising GAPDH inhibitor for suppressing breast cancer growth without obvious side effects. 18F-FDG and 18F-FLT PET/CT can noninvasively assess the levels of glycolysis and proliferation in tumors following treatment with DC-5163.
方法:体外和体内研究了DC-5163对乳腺癌细胞的作用。海马,葡萄糖摄取,乳酸生产,进行细胞ATP含量测定以检查DC-5163对细胞糖酵解的影响。细胞活力,菌落形成能力,细胞周期,和细胞凋亡通过CCK8测定进行评估,集落形成试验,流式细胞术,和免疫印迹。在小鼠乳腺癌异种移植模型中评估DC-5163的体内抗癌活性。
结果:DC-5163抑制有氧糖酵解并减少乳腺癌细胞的能量供应,从而抑制乳腺癌细胞的生长,诱导细胞周期停滞在G0/G1期,增加细胞凋亡。使用乳腺癌异种移植小鼠模型评估治疗功效。DC-5163治疗在体内显著抑制肿瘤生长而不诱导明显的全身毒性。Micro-PET/CT扫描显示,与DMSO对照组相比,DC-5163治疗组的肿瘤18F-FDG和18F-FLT摄取显著减少。
结论:我们的结果表明,DC-5163是一种有前途的GAPDH抑制剂,用于抑制乳腺癌的生长,而没有明显的副作用。18F-FDG和18F-FLTPET/CT可以无创评估DC-5163治疗后肿瘤的糖酵解和增殖水平。