METHODS: Data from 35,635 apparently healthy elderly individuals aged 60 years and above were obtained in West China Hospital from April 2020 to December 2021. We utilized the Box-Cox conversion combined with the Tukey method to normalize the data and eliminate outliers. Subgroups are divided according to gender and age to examine the division of RIs. The Z-test was used to compare differences between groups, and 95% distribution RIs were calculated using a nonparametric method.
RESULTS: In the study, we observed that the RIs for serum ferritin and Des-γ-carboxy prothrombin (DCP) were wider for men, ranging from 64.18 to 865.80 ng/ml and 14.00 to 33.00 mAU/ml, respectively, compared to women, whose ranges were 52.58 to 585.88 ng/ml and 13.00 to 29.00 mAU/ml. For other biomarkers, the overall RIs were established as follows: alpha-fetoprotein (AFP) 0-6.75 ng/ml, carcinoembryonic antigen (CEA) 0-4.85 ng/ml, carbohydrate antigen15-3 (CA15-3) for females 0-22.00 U/ml, carbohydrate antigen19-9 (CA19-9) 0-28.10 U/ml, carbohydrate antigen125 (CA125) 0-20.96 U/ml, cytokeratin 19 fragment (CYFRA21-1) 0-4.66 U/ml, neuron-specific enolase (NSE) 0-19.41 ng/ml, total and free prostate-specific antigens (tPSA and fPSA) for males 0-5.26 ng/ml and 0-1.09 ng/ml. The RIs for all these biomarkers have been validated through our rigorous processes.
CONCLUSIONS: This study preliminarily established 95% RIs for an apparently healthy elderly population in Southwestern China. Using real-world data and an indirect method, simple and reliable RIs for an elderly population can be both established and verified, which are suitable for application in various clinical laboratories.
方法:收集2020年4月至2021年12月华西医院35名60岁及以上健康老年人群的数据。我们利用Box-Cox转换与Tukey方法相结合来归一化数据并消除异常值。根据性别和年龄划分亚组以检查RI的划分。Z检验用于比较组间差异,95%分布RI是使用非参数方法计算的。
结果:在研究中,我们观察到男性血清铁蛋白和Des-γ-羧基凝血酶原(DCP)的RI更广泛,范围从64.18到865.80ng/ml和14.00到33.00mAU/ml,分别,与女性相比,其范围为52.58至585.88ng/ml和13.00至29.00mAU/ml。对于其他生物标志物,总体RI如下:甲胎蛋白(AFP)0-6.75ng/ml,癌胚抗原(CEA)0-4.85ng/ml,女性碳水化合物抗原15-3(CA15-3)0-22.00U/ml,碳水化合物抗原19-9(CA19-9)0-28.10U/ml,碳水化合物抗原125(CA125)0-20.96U/ml,细胞角蛋白19片段(CYFRA21-1)0-4.66U/ml,神经元特异性烯醇化酶(NSE)0-19.41ng/ml,男性的总和游离前列腺特异性抗原(tPSA和fPSA)为0-5.26ng/ml和0-1.09ng/ml。所有这些生物标志物的RI已经通过我们严格的过程进行了验证。
结论:本研究初步确定了中国西南地区明显健康的老年人群95%的RIs。使用真实世界的数据和间接方法,可以建立和验证老年人口的简单可靠的RI,适用于各种临床实验室。