PDF(Pubmed)
Abstract：
Circulating tumor cells (CTCs) have gathered attention as a biomarker for carcinomas. However, CTCs in sarcomas have received little attention. In this work, we investigated cell surface proteins and antibody combinations for immunofluorescence detection of sarcoma CTCs. A microfluidic device that combines filtration and immunoaffinity using gangliosides 2 and cell surface vimentin (CSV) antibodies was employed to capture CTCs. For CTC detection, antibodies against cytokeratins 7 and 8 (CK), pan-cytokeratin (panCK), or a combination of panCK and CSV were used. Thirty-nine blood samples were collected from 21 patients of various sarcoma subtypes. In the independent samples study, samples were subjected to one of three antibody combination choices. Significant difference in CTC enumeration was found between CK and panCK + CSV, and between panCK and panCK + CSV. Upon stratification of CK+ samples, those of metastatic disease had a higher CTC number than those of localized disease. In the paired samples study involving cytokeratin-positive sarcoma subtypes, using panCK antibody detected more CTCs than CK. Similarly, for osteosarcoma, using panCK + CSV combination resulted in a higher CTC count than panCK. This study emphasized deliberate selection of cell surface proteins for sarcoma CTC detection and subtype stratification for studying cancers as heterogeneous as sarcomas.
摘要：
循环肿瘤细胞（CTC）作为癌症的生物标志物已引起关注。然而,肉瘤中的CTC很少受到关注。在这项工作中,我们研究了细胞表面蛋白和抗体组合对肉瘤CTC的免疫荧光检测。使用结合过滤和使用神经节苷脂2和细胞表面波形蛋白(CSV)抗体的免疫亲和力的微流体装置用于捕获CTC。对于CTC检测，针对细胞角蛋白7和8（CK）的抗体，泛细胞角蛋白（PANCK），或使用PANCK和CSV的组合。从21例各种肉瘤亚型的患者中收集了39份血液样本。在独立样本研究中，对样品进行三种抗体组合选择之一。CK和PANCK+CSV之间的CTC计数存在显着差异，在panCK和panCK+CSV之间。在对CK+样本进行分层后，转移性疾病的CTC数量高于局部疾病.在涉及细胞角蛋白阳性肉瘤亚型的配对样本研究中，使用panCK抗体检测到比CK更多的CTC。同样，骨肉瘤，使用panCK+CSV组合导致比panCK更高的CTC计数。这项研究强调了对肉瘤CTC检测和亚型分层的细胞表面蛋白的有意选择，以研究与肉瘤不同的癌症。
