Abstract：
Posterior circulation ischemia vertigo (PCIV) is vertebrobasilar insufficiency resulting in vertigo. Banxia Baizhu Tianma Decoction (BBTD) is broadly applied to treat PCIV in China, but its efficacy and detailed mechanism remains unclear. Therefore, this study aims to investigate the effects of BBTD on PCIV, and identify important gut microbiota and its derived short-chain fatty acid (SCFA) changes and the detailed mechanism through 16 S rRNA sequencing with SCFAs profiling. In this study, the model of PCIV was established by surgical ligation of the right subclavian artery (RSCA) and right common carotid artery (RCCA). We found that BBTD administration effectively reduced the volume of cerebral infarction and improved neurologic functions, reduced neuronal apoptosis and neuroinflammatory. Moreover, BBTD significantly modulated the diversity and composition of the gut microbiota, including increasing the relative abundance of Lactobacillus, Prevotella and Akkermansia and decreasing relative abundances of Lachnospiraceae, Bacteroidetes (S24-7) and Ruminococcaceae. BBTD treatment also increased propionate content. Propionate mediates the the recovery of neurological functions and anti-apoptotic effects of BBTD in PCIV rat. Our findings wish to discover the potential mechanism of BBTD treatment on PCIV and promote its clinical application.
摘要：
后循环缺血性眩晕（PCIV）是椎基底动脉供血不足导致的眩晕。半夏白术天麻汤(BBTD)在我国广泛应用于PCIV,但其疗效和详细机制尚不清楚。因此,本研究旨在探讨BBTD对PCIV的影响，并通过16SrRNA测序和SCFAs谱鉴定重要的肠道菌群及其衍生的短链脂肪酸（SCFA）变化和详细机制。在这项研究中,通过手术结扎右锁骨下动脉（RSCA）和右颈总动脉（RCCA）建立PCIV模型。我们发现BBTD给药可有效减少脑梗死的体积，改善神经功能，减少神经元凋亡和神经炎症。此外，BBTD显著调节肠道微生物群的多样性和组成，包括增加乳酸菌的相对丰度，Prevotella和Akkermansia以及Lachnospiraceae的相对丰度降低，拟杆菌（S24-7）和反刍动物科。BBTD处理也增加了丙酸盐含量。丙酸盐介导BBTD对PCIV大鼠神经功能的恢复和抗凋亡作用。我们的发现希望发现BBTD治疗PCIV的潜在机制并促进其临床应用。
