PDF(Pubmed)
Abstract：
UNASSIGNED: The rat controlled elevation of intraocular pressure (CEI) model allows study of in vivo responses to short-term exposure to defined intraocular pressures (IOP). In this study, we used NanoString technology to investigate in vivo IOP-related gene responses in the trabecular meshwork (TM) and optic nerve head (ONH) simultaneously from the same animals.
UNASSIGNED: Male and female rats (N = 35) were subjected to CEI for 8 hours at pressures simulating mean, daytime normotensive rat IOP (CEI-20), or 2.5× IOP (CEI-50). Naïve animals that received no anesthesia or surgical interventions served as controls. Immediately after CEI, TM and ONH tissues were dissected, RNA was isolated, and samples were analyzed with a NanoString panel containing 770 genes. Postprocessing, raw count data were uploaded to ROSALIND for differential gene expression analyses.
UNASSIGNED: For the TM, 45 IOP-related genes were significant in the CEI-50 versus CEI-20 and CEI-50 versus naïve comparisons, with 15 genes common to both comparisons. Bioinformatics analysis identified Notch and transforming growth factor beta (TGFβ) pathways to be the most up- and downregulated Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, respectively. For ONH, 22 significantly differentially regulated genes were identified in the CEI-50 versus naïve comparison. Pathway analysis identified defense response and immune response as two significantly upregulated biological process pathways.
UNASSIGNED: This study demonstrated the ability to assay short-term IOP-responsive genes in both TM and ONH tissues simultaneously. In the TM, downregulation of TGFβ pathway genes suggests that TM responses may reduce TGFβ-induced extracellular matrix synthesis. For ONH, the initial response to short-term elevated IOP may be protective.
UNASSIGNED: Male and female rats (N = 35) were subjected to CEI for 8 hours at pressures simulating mean, daytime normotensive rat IOP (CEI-20), or 2.5× IOP (CEI-50). Naïve animals that received no anesthesia or surgical interventions served as controls. Immediately after CEI, TM and ONH tissues were dissected, RNA was isolated, and samples were analyzed with a NanoString panel containing 770 genes. Postprocessing, raw count data were uploaded to ROSALIND for differential gene expression analyses.
UNASSIGNED: For the TM, 45 IOP-related genes were significant in the CEI-50 versus CEI-20 and CEI-50 versus naïve comparisons, with 15 genes common to both comparisons. Bioinformatics analysis identified Notch and transforming growth factor beta (TGFβ) pathways to be the most up- and downregulated Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, respectively. For ONH, 22 significantly differentially regulated genes were identified in the CEI-50 versus naïve comparison. Pathway analysis identified defense response and immune response as two significantly upregulated biological process pathways.
UNASSIGNED: This study demonstrated the ability to assay short-term IOP-responsive genes in both TM and ONH tissues simultaneously. In the TM, downregulation of TGFβ pathway genes suggests that TM responses may reduce TGFβ-induced extracellular matrix synthesis. For ONH, the initial response to short-term elevated IOP may be protective.
摘要：
■大鼠控制的眼内压升高（CEI）模型允许研究对短期暴露于定义的眼内压（IOP）的体内反应。在这项研究中,我们使用NanoString技术研究了来自同一动物的小梁网（TM）和视神经头（ONH）中与体内IOP相关的基因反应。
■雄性和雌性大鼠（N=35）在模拟平均压力下接受CEI8小时，白天血压正常的大鼠眼压（CEI-20），或2.5×IOP（CEI-50）。未接受麻醉或手术干预的幼稚动物作为对照。在CEI之后，解剖TM和ONH组织，RNA被分离，用含有770个基因的NanoString面板分析样品。后处理,将原始计数数据上传到ROSALIND用于差异基因表达分析。
■对于TM，45个IOP相关基因在CEI-50与CEI-20和CEI-50与初始比较中是显著的，两种比较共有15个基因。生物信息学分析确定Notch和转化生长因子β（TGFβ）途径是最上调和下调的京都基因和基因组百科全书（KEGG）途径，分别。对于ONH，在CEI-50与初始比较中鉴定出22个显著差异调节的基因。路径分析确定防御反应和免疫反应为两个显著上调的生物过程途径。
■这项研究证明了在TM和ONH组织中同时测定短期IOP响应基因的能力。在TM中,TGFβ通路基因的下调表明TM反应可能减少TGFβ诱导的细胞外基质合成。对于ONH，对短期IOP升高的初始反应可能是保护性的.
■雄性和雌性大鼠（N=35）在模拟平均压力下接受CEI8小时，白天血压正常的大鼠眼压（CEI-20），或2.5×IOP（CEI-50）。未接受麻醉或手术干预的幼稚动物作为对照。在CEI之后，解剖TM和ONH组织，RNA被分离，用含有770个基因的NanoString面板分析样品。后处理,将原始计数数据上传到ROSALIND用于差异基因表达分析。
■对于TM，45个IOP相关基因在CEI-50与CEI-20和CEI-50与初始比较中是显著的，两种比较共有15个基因。生物信息学分析确定Notch和转化生长因子β（TGFβ）途径是最上调和下调的京都基因和基因组百科全书（KEGG）途径，分别。对于ONH，在CEI-50与初始比较中鉴定出22个显著差异调节的基因。路径分析确定防御反应和免疫反应为两个显著上调的生物过程途径。
■这项研究证明了在TM和ONH组织中同时测定短期IOP响应基因的能力。在TM中,TGFβ通路基因的下调表明TM反应可能减少TGFβ诱导的细胞外基质合成。对于ONH，对短期IOP升高的初始反应可能是保护性的.
