Abstract：
The aim of the study was to investigate the effect of the immunostimulant Mycobacterium Cell Wall Fraction (MCWF) on the treatment of S. aureus SCM by intravenous application. The study included 45 HF dairy cows in 2nd and 3rd month after parturition divided into three groups (n = 15 per group): the MC + group - cows with S. aureus SCM treated with MCWF; the MC- group - cows with S. aureus SCM, with no treatment; and the C group - the control group of healthy cow with no treatment. Samples were collected 0th (I sample), 7th (II), and 14th day (III) from the day of SCM diagnosis and on day 21st (IV). A greater influx of leukocytes was confirmed into milk after 7 days after MCWF treatment in MC + group, which was followed by increase of WBC and LYM in blood. These results support the hypothesis of effective action of MCWF, and in quarters with lower-grade infection, bacteriological cure was achieved. The MC- group had a statistically higher concentration of TBARS and CAT activity in milk, while MC + group had lower blood serum LDH activity, which indicates a positive effect of the MCWF application and a lower exposure of the tissue to lipide peroxidation and inflammation caused by S. aureus. The application of MCWF would give new possibilities in the prevention and therapy of mammary gland diseases without fear of the presence of residues and the emergence of bacterial resistance. In future studies, the effects of local and systemic application of MCWF in the treatment of S. aureus SCM should be compared.
摘要：
该研究的目的是研究免疫刺激剂分枝杆菌细胞壁部分（MCWF）对静脉应用金黄色葡萄球菌SCM的治疗效果。该研究包括分娩后第2个月和第3个月的45头HF奶牛，分为三组（每组n=15）：MC组-用MCWF处理的金黄色葡萄球菌SCM奶牛；MC-组-金黄色葡萄球菌SCM奶牛，不治疗；C组-健康奶牛对照组不治疗。第0次收集样品(I样品)，第七(二),和SCM诊断当天的第14天(III)和第21天(IV)。MCWF治疗后7天，MC+组证实有更多的白细胞流入牛奶，其次是血液中WBC和LYM的增加。这些结果支持MCWF有效作用的假设，在低度感染的地方，实现了细菌学治愈。MC-组有统计学上较高的浓度的TBARS和CAT活性，而MC+组有较低的血清LDH活性，这表明MCWF应用的积极作用和较低的组织暴露于由金黄色葡萄球菌引起的脂质过氧化和炎症。MCWF的应用将为预防和治疗乳腺疾病提供新的可能性，而不必担心残留物的存在和细菌耐药性的出现。在未来的研究中,应比较局部和全身应用MCWF治疗金黄色葡萄球菌SCM的效果。
