Abstract：
This study evaluates the diagnostic efficacy of urinary biomarkers, Neutrophil Gelatinase-Associated Lipocalin (uNGAL), and Kidney Injury Molecule-1 (uKIM-1), in identifying Acute Kidney Injury (AKI) in dogs affected with leptospirosis or babesiosis. Acute kidney injury was diagnosed based on the increase in serum creatinine levels above 0.3 mg/dL within 48 h and dogs were categorized according to AKI grades based on International Renal Interest Society guidelines. Traditional biomarkers (serum creatinine and blood urea nitrogen) and novel biomarkers like urinary NGAL and urinary KIM-1 levels were measured and compared to concentrations obtained in control dogs. Statistical analysis assessed significant differences (P < 0.01) across AKI grades, specifically noting elevated urinary NGAL and KIM-1 in IRIS grade I AKI (P < 0.001). The study highlights the diagnostic significance of urinary NGAL and KIM-1 as early indicators of renal damage, particularly valuable in non-azotemic AKI cases, offering promising markers for early AKI diagnosis in veterinary clinical settings. These biomarkers demonstrate clinical utility and underscore their potential for improving AKI management in veterinary medicine. Further validation studies involving larger cohorts and diverse etiologies of AKI are needed to confirm the diagnostic accuracy and clinical utility of urinary NGAL and KIM-1 in veterinary practice.
摘要：
这项研究评估了尿液生物标志物的诊断功效，中性粒细胞明胶酶相关脂质运载蛋白（uNGAL），和肾损伤分子-1（uKIM-1），识别患有钩端螺旋体病或巴贝斯虫病的狗的急性肾损伤（AKI）。根据48小时内血清肌酐水平高于0.3mg/dL的增加诊断急性肾损伤，并根据国际肾脏兴趣协会指南的AKI等级对狗进行分类。测量传统生物标志物(血清肌酐和血尿素氮)和新的生物标志物如尿NGAL和尿KIM-1水平,并与在对照狗中获得的浓度进行比较。统计分析评估了AKI等级之间的显着差异（P<0.01），特别注意IRISI级AKI中尿NGAL和KIM-1升高(P<0.001)。该研究强调了尿NGAL和KIM-1作为肾损害的早期指标的诊断意义，在非氮血症性AKI病例中特别有价值，为兽医临床中的早期AKI诊断提供有希望的标志物。这些生物标志物显示了临床实用性，并强调了它们在兽医学中改善AKI管理的潜力。需要涉及更大的队列和AKI的不同病因的进一步验证研究，以确认尿NGAL和KIM-1在兽医实践中的诊断准确性和临床实用性。
