Abstract：
Polysaccharides from natural sources can regulate the composition of intestinal flora through the \"gut-liver axis\" pathway, potentially ameliorating alcoholic liver injury. Aspalathus linearis, also known as rooibos, is one such natural product that has shown promise in this regard. This study looked at the structural properties of A. linearis polysaccharide (ALP) and how well it would work to treat acute alcoholic liver impairment. This study looks at the composition of monosaccharides, functional groups, and molecular weight (Mw) of a newly discovered water-soluble polysaccharide, named ALP. The polysaccharide is composed of pyranose rings, amide groups, and sulfate groups linked by β-glycosidic linkage. It has a relative Mw of 4.30 × 103 kDa and is composed of glucose, rhamnose, and some other monosaccharides. The study found that treating mice with the model of acute alcoholic liver disease with ALP could alleviate pathological symptoms, inhibit the release of inflammatory cytokines, and suppress indicators of oxidative stress. Experiments have shown that different doses of ALP can activate the P4502E1/Keap1-Nrf2-HO-1 signaling pathway. The regulation of inflammatory factors and downstream antioxidant enzymes occurs as a result. Based on these data, it is likely that ALP protects the liver via the \"gut-liver axis\" pathway by reducing oxidative stress-related damage, inflammation, and alcohol-related alterations to the gut microbiome. The results indicate that ALP mitigates injury caused by oxidative stress, inflammatory responses, and changes in the gut microbiota induced by alcohol through the \"gut-liver axis\" pathway, which provides protection to the liver. This provides preliminary evidence for the development of related drugs. PRACTICAL APPLICATION: Researchers extracted a polysaccharide from fresh leaves of Auricularia auricula. The polysaccharide was purified and determined to have a predominantly homogeneous molecular weight. An acute alcoholic liver damage mouse model was established, and it was concluded that the polysaccharide could ameliorate liver injury in mice through the \"gut-liver axis\" pathway. This novel polysaccharide can be used as an additive to develop functional foods with beneficial effects, which can positively impact the daily maintenance of consumers.
摘要：
天然来源的多糖可以通过“肠-肝轴”途径调节肠道菌群的组成，可能改善酒精性肝损伤。Aspalathuslinearis,也被称为路易波斯，是一种在这方面显示出希望的天然产品。这项研究观察了A.linearis多糖（ALP）的结构特性以及它对治疗急性酒精性肝损害的作用。这项研究着眼于单糖的组成，功能组，和新发现的水溶性多糖的分子量（Mw），名为ALP。多糖由吡喃糖环组成，酰胺基团，和硫酸基团通过β-糖苷键连接。它的相对Mw为4.30×103kDa，由葡萄糖组成，鼠李糖,和其他一些单糖。研究发现,ALP治疗小鼠急性酒精性肝病模型可减轻病理症状,抑制炎症细胞因子的释放，并抑制氧化应激指标。实验表明,不同剂量的ALP可以激活P4502E1/Keap1-Nrf2-HO-1信号通路。炎症因子和下游抗氧化酶的调节由此发生。基于这些数据,ALP可能通过减少氧化应激相关损伤，通过“肠-肝轴”途径保护肝脏，炎症,和酒精相关的肠道微生物组的改变。结果表明，ALP减轻了氧化应激引起的损伤，炎症反应，以及酒精通过“肠-肝轴”途径诱导的肠道微生物群的变化，为肝脏提供保护。这为相关药物的开发提供了初步证据。实际应用：研究人员从黑木耳的新鲜叶中提取了一种多糖。将多糖纯化并测定为具有主要均一的分子量。建立急性酒精性肝损伤小鼠模型,结论是多糖可以通过“肠-肝轴”途径改善小鼠的肝损伤。这种新型多糖可用作添加剂，开发具有有益效果的功能性食品，这可以积极影响消费者的日常维护。
