UNASSIGNED: Twenty-eight rats were divided into eight groups using the BBFN bilaterally (the left nerve was used for PBM), namely: G1 - control group, right BBFN (without injury); G2 - control group, left BBFN (without injury + PBM); G3 - Denervated right BBFN (neurotmesis); G4 - Denervated left BBFN (neurotmesis + PBM); G5 - Immediate repair of right BBFN (neurotmesis + HFB); G6 - Immediate repair of left BBFN (neurotmesis + HFB + PBM); G7 - Delayed repair of right BBFN (neurotmesis + HFB); G8 - Delayed repair of left BBFN (neurotmesis + HFB + PBM). Delayed repair occurred after two weeks of denervation. All animals were sacrificed after six weeks postoperatively.
UNASSIGNED: In the parameters of the BBFN, we observed inferior results in the groups with delayed repair, in relation to the groups with immediate repair, with a significant difference (p < 0.05) in the diameter of the nerve fiber, the axon, and the thickness of the myelin sheath of the group with immediate repair with PBM compared to the other experimental groups. In measuring the muscle fiber area, groups G7 (826.4 ± 69.90) and G8 (836.7 ± 96.44) were similar to G5 (882.8 ± 70.51). In the functional analysis, the G7 (4.10 ± 0.07) and G8 (4.12 ± 0.08) groups presented normal parameters.
UNASSIGNED: We demonstrated that delayed repair of BBFN is possible with HFB, but with worse results compared to immediate repair, and that PBM has a positive influence on nerve regeneration results in immediate repair.
■使用双侧BBFN将28只大鼠分为8组(左神经用于PBM),即:G1-对照组,右侧BBFN(无损伤);G2-对照组,左BBFN(无损伤+PBM);G3-右BBFN(神经损伤+PBM);G4-左BBFN(神经损伤+PBM);G5-右BBFN立即修复(神经损伤+HFB);G6-左BBFN立即修复(神经损伤+HFB+PBM)去神经两周后发生延迟修复。术后6周后处死所有动物。
■在BBFN的参数中,我们观察到延迟修复组的效果较差,关于立即修复的团体,神经纤维直径有显著差异(p<0.05),轴突,与其他实验组相比,PBM立即修复组的髓鞘厚度。在测量肌纤维面积时,G7组(826.4±69.90)和G8组(836.7±96.44)与G5组(882.8±70.51)相似。在功能分析中,G7(4.10±0.07)和G8(4.12±0.08)组呈现正常参数。
■我们证明了用HFB可以延迟修复BBFN,但是与立即修复相比效果更差,并且PBM对神经再生有积极影响,导致立即修复。