PDF(Pubmed)
Abstract：
UNASSIGNED: Restoration of blood supply is a desired goal for the treatment of acute ischemic stroke. However, the restoration often leads to cerebral ischemia-reperfusion injury (CIR/I), which greatly increases the risk of non-neural organ damage. In particular, the acute kidney injury might be one of the most common complications.
UNASSIGNED: The study aimed to understand the damage occurred and the potential molecular mechanisms.
UNASSIGNED: The study was explored on the CIR/I rats generated by performing middle cerebral artery occlusion/reperfusion (MCAO/Reperfusion). The rats were evaluated with injury on the brains, followed by the non-neural organs including kidneys, livers, colons and stomachs. They were examined further with histopathological changes, and gene expression alterations by using RT-qPCR of ten aquaporins (Aqps) subtypes including Aqp1～Aqp9 and Aqp11. Furthermore, the Aqps expression profiles were constructed for each organ and analyzed by performing Principle Component Analysis. In addition, immunohistochemistry was explored to look at the protein expression of Aqp1, Aqp2, Aqp3 and Aqp4 in the rat kidneys.
UNASSIGNED: There was a prominent down-regulation profile in the MCAO/Reperfusion rat kidneys. The protein expression of Aqp1, Aqp2, Aqp3 and Aqp4 was decreased in the kidneys of the MCAO/Reperfusion rats. We suggested that the kidney was in the highest risk to be damaged following the CIR/I. Down-regulation of Aqp2, Aqp3 and Aqp4 was involved in the acute kidney injury induced by the CIR/I.
UNASSIGNED: The study aimed to understand the damage occurred and the potential molecular mechanisms.
UNASSIGNED: The study was explored on the CIR/I rats generated by performing middle cerebral artery occlusion/reperfusion (MCAO/Reperfusion). The rats were evaluated with injury on the brains, followed by the non-neural organs including kidneys, livers, colons and stomachs. They were examined further with histopathological changes, and gene expression alterations by using RT-qPCR of ten aquaporins (Aqps) subtypes including Aqp1～Aqp9 and Aqp11. Furthermore, the Aqps expression profiles were constructed for each organ and analyzed by performing Principle Component Analysis. In addition, immunohistochemistry was explored to look at the protein expression of Aqp1, Aqp2, Aqp3 and Aqp4 in the rat kidneys.
UNASSIGNED: There was a prominent down-regulation profile in the MCAO/Reperfusion rat kidneys. The protein expression of Aqp1, Aqp2, Aqp3 and Aqp4 was decreased in the kidneys of the MCAO/Reperfusion rats. We suggested that the kidney was in the highest risk to be damaged following the CIR/I. Down-regulation of Aqp2, Aqp3 and Aqp4 was involved in the acute kidney injury induced by the CIR/I.
摘要：
■恢复血液供应是治疗急性缺血性中风的理想目标。然而,修复常导致脑缺血再灌注损伤(CIR/I),这大大增加了非神经器官损伤的风险。特别是,急性肾损伤可能是最常见的并发症之一。
■该研究旨在了解发生的损伤和潜在的分子机制。
■对通过进行大脑中动脉闭塞/再灌注（MCAO/再灌注）产生的CIR/I大鼠进行了研究。评估大鼠的大脑损伤，其次是包括肾脏在内的非神经器官，肝脏,结肠和胃.他们进一步检查了组织病理学变化，通过RT-qPCR对包括Aqp1〜Aqp9和Aqp11在内的十种水通道蛋白（Aqps）亚型进行基因表达改变。此外，构建每个器官的Aqps表达谱，并通过主成分分析进行分析。此外,采用免疫组织化学方法观察Aqp1、Aqp2、Aqp3和Aqp4在大鼠肾脏中的蛋白表达。
■在MCAO/再灌注大鼠肾脏中存在显著的下调特征。MCAO/再灌注大鼠肾脏中Aqp1、Aqp2、Aqp3和Aqp4的蛋白表达降低。我们建议肾脏在CIR/I后受损的风险最高。Aqp2，Aqp3和Aqp4的下调参与了CIR/I引起的急性肾损伤。
■该研究旨在了解发生的损伤和潜在的分子机制。
■对通过进行大脑中动脉闭塞/再灌注（MCAO/再灌注）产生的CIR/I大鼠进行了研究。评估大鼠的大脑损伤，其次是包括肾脏在内的非神经器官，肝脏,结肠和胃.他们进一步检查了组织病理学变化，通过RT-qPCR对包括Aqp1〜Aqp9和Aqp11在内的十种水通道蛋白（Aqps）亚型进行基因表达改变。此外，构建每个器官的Aqps表达谱，并通过主成分分析进行分析。此外,采用免疫组织化学方法观察Aqp1、Aqp2、Aqp3和Aqp4在大鼠肾脏中的蛋白表达。
■在MCAO/再灌注大鼠肾脏中存在显著的下调特征。MCAO/再灌注大鼠肾脏中Aqp1、Aqp2、Aqp3和Aqp4的蛋白表达降低。我们建议肾脏在CIR/I后受损的风险最高。Aqp2，Aqp3和Aqp4的下调参与了CIR/I引起的急性肾损伤。
