PDF(Pubmed)
Abstract：
Overexpression of Glycine max disease resistant 1 (GmDR1) exhibits broad-spectrum resistance against Fusarium virguliforme, Heterodera glycines (soybean cyst nematode), Tetranychus urticae (Koch) (spider mites), and Aphis glycines Matsumura (soybean aphids) in soybean. To understand the mechanisms of broad-spectrum immunity mediated by GmDR1, the transcriptomes of a strong and a weak GmDR1-overexpressor following treatment with chitin, a pathogen- and pest-associated molecular pattern (PAMP) common to these organisms, were investigated. The strong and weak GmDR1-overexpressors exhibited altered expression of 6098 and 992 genes, respectively, as compared to the nontransgenic control following chitin treatment. However, only 192 chitin- and 115 buffer-responsive genes exhibited over two-fold changes in expression levels in both strong and weak GmDR1-overexpressors as compared to the control. MapMan analysis of the 192 chitin-responsive genes revealed 64 biotic stress-related genes, of which 53 were induced and 11 repressed as compared to the control. The 53 chitin-induced genes include nine genes that encode receptor kinases, 13 encode nucleotide-binding leucine-rich repeat (NLR) receptor proteins, seven encode WRKY transcription factors, four ethylene response factors, and three MYB-like transcription factors. Investigation of a subset of these genes revealed three receptor protein kinases, seven NLR proteins, and one WRKY transcription factor genes that are induced following F. virguliforme and H. glycines infection. The integral plasma membrane GmDR1 protein most likely recognizes PAMPs including chitin and activates transcription of genes encoding receptor kinases, NLR proteins and defense-related genes. GmDR1 could be a pattern recognition receptor that regulates the expression of several NLRs for expression of PAMP-triggered immunity and/or priming the effector triggered immunity.
摘要：
大豆抗病1（GmDR1）的过表达表现出对镰刀菌的广谱抗性，异端甘氨酸（大豆胞囊线虫），荨麻疹（Koch）（蜘蛛螨），和大豆中的Aphis甘氨酸Matsumura（大豆蚜虫）。为了了解GmDR1介导的广谱免疫的机制，用几丁质处理后的强和弱GmDR1过表达的转录组，这些生物常见的病原体和害虫相关分子模式(PAMP)，被调查了。强和弱的GmDR1过表达显示6098和992个基因的表达改变，分别,与几丁质处理后的非转基因对照相比。然而,与对照相比，只有192个几丁质和115个缓冲响应基因在强和弱GmDR1过表达基因中的表达水平变化超过两倍。MapMan分析192个几丁质响应基因揭示了64个生物胁迫相关基因，与对照组相比，其中53例被诱导，11例被抑制。这53个几丁质诱导的基因包括9个编码受体激酶的基因，13编码核苷酸结合富含亮氨酸的重复序列（NLR）受体蛋白，七个编码WRKY转录因子，四个乙烯响应因素，和三个MYB样转录因子。对这些基因的一个子集的研究揭示了三种受体蛋白激酶，七种NLR蛋白，和一个WRKY转录因子基因在F.virguliforme和H.glycines感染后诱导。整合的质膜GmDR1蛋白最有可能识别PAMPs，包括几丁质，并激活编码受体激酶的基因的转录，NLR蛋白和防御相关基因。GmDR1可能是一种模式识别受体，可调节几种NLR的表达，以表达PAMP触发的免疫和/或引发效应子触发的免疫。
