Abstract：
The objective for this study is to advance the development of a specialized biomaterial that can effectively facilitate the regeneration of adipose tissue. In prior studies, the assessment of collagen (Col), elastin (Ela), and fibrin (Fib) unary scaffolds has been conducted. However, it is important to note that native adipose tissue is comprised of a diverse array of extracellular matrix (ECM) constituents. To mimic this behavior, binary compositions of collagen, elastin, and fibrin are fabricated in a 1:1 ratio, resulting in the formation of Col/Ela, Col/Fib, and Ela/Fib composites through a customized fabrication procedure. The physical properties of these scaffolds are comprehensively analyzed using a range of material characterization techniques. Additionally, the biological properties of the scaffolds are investigated by examining the survival, proliferation, and phenotype of adipose-derived stem cells. Subsequently, the aforementioned binary scaffolds are implanted into a rodent model for 28 days. the explants are analysed through X-ray microtomography, histology, and immunohistochemistry. The findings of the study demonstrate that the utilization of binary combinations of Col/Ela, Col/Fib, and Ela/Fib has a discernible impact on the physical and biological characteristics of the scaffolds. Nevertheless, Ela/Fib exhibits characteristics that make it a suitable candidate for adipogenesis due to its notable upregulation of caveolin-1 expression in both acellular and cellular cohorts. The combination of two natural polymers in this cell-material interaction has significantly enhanced the comprehension of adipogenesis.
摘要：
用于靶向组织再生的最佳医疗保健生物材料的开发提出了重大挑战。我们目前的目标是推进能够有效促进脂肪组织再生的专用生物材料的开发。在先前的研究中，胶原蛋白的评估，弹性蛋白,和纤维蛋白一元支架已经进行。然而,重要的是要注意,天然脂肪组织由多种细胞外基质(ECM)成分组成。为了模仿这种行为，我们制造了胶原蛋白的二元组合物，弹性蛋白,和纤维蛋白的比例为1:1，导致Col/Ela的形成，Col/Fib，和Ela/Fib复合材料通过定制的制造程序。使用一系列材料表征技术全面分析了这些支架的物理性质。此外,通过检查支架的存活率来研究支架的生物学特性，扩散,和脂肪干细胞的表型。随后，将上述二元支架引入活体啮齿动物模型,持续28天.在这个时期之后,外植体通过X射线显微断层扫描进行分析，组织学,和免疫组织化学，以评估它们的免疫相容性，一体化,和脂肪生成。研究结果表明，利用Col/Ela二元组合，Col/Fib，和Ela/Fib对支架的物理和生物学特性有明显的影响。然而，Ela/Fib表现出的特征使其成为脂肪生成的合适候选者，因为它在无细胞和细胞队列中均显着上调了caveolin-1的表达。在这种细胞-物质相互作用中两种天然聚合物的组合显着增强了我们对脂肪生成的理解。本文受版权保护。保留所有权利。
