PDF(Pubmed)
Abstract：
The benzophenone (BP) family, including oxybenzone (BP-3), a prevalent sunscreen ingredient and environmental contaminant, has raised concerns since the year 2005. This study investigated oxybenzone toxicity in zebrafish (Danio rerio) eleutheroembryos and brine shrimp (Artemia salina) nauplii, focusing on the LC50 and developmental impacts. Zebrafish embryos (0.100-1.50 mg/L BP-3, 96 h) and A. salina (0.100-5.00 mg/L BP-3, 48 h) were tested with ultrasound-assisted emulsified liquid-phase microextraction (UA-ELPME) used for zebrafish tissue analysis. HPLC-DAD determined BP-3 concentrations (highest: 0.74 ± 0.13 mg/L). Although no significant zebrafish embryo mortality or hatching changes occurred, developmental effects were evident. Lethal concentrations were determined (A. salina LC50 at 24 h = 3.19 ± 2.02 mg/L; D. rerio embryos LC50 at 24 h = 4.19 ± 3.60 mg/L), with malformations indicating potential teratogenic effects. A. salina displayed intestinal tract alterations and D. rerio embryos exhibited pericardial edema and spinal deformities. These findings highlight oxybenzone\'s environmental risks, posing threats to species and ecosystem health.
摘要：
二苯甲酮（BP）家族，包括氧苯酮（BP-3），一种普遍的防晒成分和环境污染物，自2005年以来一直引起人们的关注。这项研究调查了斑马鱼（Daniorerio）eleutheroemes和盐水虾（Artemiasalina）无节幼体中的氧苯酮毒性，关注LC50和发展影响。采用超声辅助乳化液相微萃取法(UA-ELPME)检测斑马鱼胚胎(0.100-1.50mg/LBP-3,96h)和盐藻(0.100-5.00mg/LBP-3,48h)。HPLC-DAD测定BP-3浓度(最高:0.74±0.13mg/L)。虽然没有显著的斑马鱼胚胎死亡或孵化变化发生，发育效果明显。确定致死浓度（A.24h时的盐藻LC50=3.19±2.02mg/L；D.Rerio胚胎24h时的LC50=4.19±3.60mg/L），畸形表明潜在的致畸作用。A.salina表现出肠道改变，D.rerio胚胎表现出心包水肿和脊柱畸形。这些发现突出了氧苯酮的环境风险，对物种和生态系统健康构成威胁。
