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Abstract：
Methicillin-resistant Staphylococcus aureus (MRSA) is a predominant nosocomial infection-causing bacteria. The aim of this study was to develop a novel single-bacteria multiplex digital PCR assays (SMD-PCR), which is capable of simultaneously detecting and discriminating Methicillin-sensitive Staphylococcus aureus (MSSA) and MRSA. This protocol employed TaqMan probes to detect SAOUHSC_00106 and mecA genes, with the latter being linked to methicillin resistance. A total of 72 samples from various specimen types were evaluated. The accuracy rates for the sputum samples, pus samples, swab samples, ear secretion samples, and catheter samples were 94.44%, 100%, 92%, 100%, and 100%, respectively. Our results showed that the clinical practicability of SMD-PCR has applicability to the rapid detection of MRSA without DNA extraction or bacterial culture, and can be utilized for the rapid detection of Staphylococcus aureus and the timely identification of MRSA in clinical samples, thereby providing an advanced platform for the rapid diagnosis of clinical MRSA infection.
摘要：
耐甲氧西林金黄色葡萄球菌（MRSA）是引起医院感染的主要细菌。本研究的目的是开发一种新型的单细菌多重数字PCR检测方法（SMD-PCR），能够同时检测和区分甲氧西林敏感金黄色葡萄球菌（MSSA）和MRSA。该协议采用TaqMan探针检测SAOUHSC_00106和mecA基因，后者与甲氧西林耐药性有关。评估了来自各种样品类型的总共72个样品。痰样本的准确率，脓液样本,拭子样本,耳朵分泌物样本，导管样本为94.44%,100%,92%,100%,100%,分别。我们的结果表明，SMD-PCR的临床实用性适用于不需要DNA提取或细菌培养的MRSA的快速检测，可用于金黄色葡萄球菌的快速检测和临床样品中MRSA的及时鉴定,从而为临床MRSA感染的快速诊断提供了先进的平台。
