PDF(Pubmed)
Abstract：
UNASSIGNED: In recent years, the world\'s attention has been drawn to antimicrobial resistance (AMR) because to the frightening prospect of growing death rates. Nanomaterials are being investigated due to their potential in a wide range of technical and biological applications.
UNASSIGNED: The purpose of this study was to biosynthesis zinc oxide nanoparticles (ZnONPs) using Aspergillus sp. SA17 fungal extract, followed by characterization of the produced nanoparticles (NP) using electron microscopy (TEM and SEM), UV-analysis, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR).
UNASSIGNED: The HR-TEM revealed spherical nanoparticles with an average size of 7.2 nm, and XRD validated the crystalline nature and crystal structure features of the generated ZnONPs, while the zeta potential was 18.16 mV, indicating that the particles\' surfaces are positively charged. The FT-IR was also used to identify the biomolecules involved in the synthesis of ZnONPs. The antibacterial and anticancer properties of both the crude fungal extract and its nano-form against several microbial strains and cancer cell lines were also investigated. Inhibition zone diameters against pathogenic bacteria ranged from 3 to 13 mm, while IC50 values against cancer cell lines ranged from 17.65 to 84.55 M. Additionally, 33 compounds, including flavonoids, phenolic acids, coumarins, organic acids, anthraquinones, and lignans, were discovered through chemical profiling of the extract using UPLC-QTOF-MS/MS. Some molecules, such pomiferin and glabrol, may be useful for antibacterial purposes, according to in silico study, while daidzein 4\'-sulfate showed promise as an anti-cancer metabolite.
UNASSIGNED: The purpose of this study was to biosynthesis zinc oxide nanoparticles (ZnONPs) using Aspergillus sp. SA17 fungal extract, followed by characterization of the produced nanoparticles (NP) using electron microscopy (TEM and SEM), UV-analysis, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR).
UNASSIGNED: The HR-TEM revealed spherical nanoparticles with an average size of 7.2 nm, and XRD validated the crystalline nature and crystal structure features of the generated ZnONPs, while the zeta potential was 18.16 mV, indicating that the particles\' surfaces are positively charged. The FT-IR was also used to identify the biomolecules involved in the synthesis of ZnONPs. The antibacterial and anticancer properties of both the crude fungal extract and its nano-form against several microbial strains and cancer cell lines were also investigated. Inhibition zone diameters against pathogenic bacteria ranged from 3 to 13 mm, while IC50 values against cancer cell lines ranged from 17.65 to 84.55 M. Additionally, 33 compounds, including flavonoids, phenolic acids, coumarins, organic acids, anthraquinones, and lignans, were discovered through chemical profiling of the extract using UPLC-QTOF-MS/MS. Some molecules, such pomiferin and glabrol, may be useful for antibacterial purposes, according to in silico study, while daidzein 4\'-sulfate showed promise as an anti-cancer metabolite.
摘要：
■近年来，世界的注意力已经被吸引到抗菌素耐药性（AMR），因为死亡率增长的可怕前景。由于纳米材料在广泛的技术和生物应用中的潜力，正在进行研究。
■这项研究的目的是使用曲霉属生物合成氧化锌纳米颗粒（ZnONPs）。SA17真菌提取物，然后用电子显微镜(TEM和SEM)表征所产生的纳米粒子(NP)，紫外线分析，X射线衍射(XRD)傅里叶变换红外光谱(FT-IR)。
■HR-TEM显示平均尺寸为7.2nm的球形纳米颗粒，和XRD验证了生成的ZnONPs的结晶性质和晶体结构特征，而zeta电位为18.16mV，表明颗粒表面带正电。FT-IR也用于鉴定参与ZnONPs合成的生物分子。还研究了粗真菌提取物及其纳米形式对几种微生物菌株和癌细胞系的抗菌和抗癌特性。针对病原菌的抑制区直径范围为3至13毫米，而针对癌细胞系的IC50值范围为17.65至84.55M。此外，33个化合物，包括类黄酮,酚酸，香豆素,有机酸,蒽醌,和木脂素，通过使用UPLC-QTOF-MS/MS对提取物进行化学分析发现。一些分子，比如波弗林和glabrol，可能用于抗菌目的，根据计算机模拟研究，而大豆苷元4'-硫酸盐显示出作为抗癌代谢产物的前景。
■这项研究的目的是使用曲霉属生物合成氧化锌纳米颗粒（ZnONPs）。SA17真菌提取物，然后用电子显微镜(TEM和SEM)表征所产生的纳米粒子(NP)，紫外线分析，X射线衍射(XRD)傅里叶变换红外光谱(FT-IR)。
■HR-TEM显示平均尺寸为7.2nm的球形纳米颗粒，和XRD验证了生成的ZnONPs的结晶性质和晶体结构特征，而zeta电位为18.16mV，表明颗粒表面带正电。FT-IR也用于鉴定参与ZnONPs合成的生物分子。还研究了粗真菌提取物及其纳米形式对几种微生物菌株和癌细胞系的抗菌和抗癌特性。针对病原菌的抑制区直径范围为3至13毫米，而针对癌细胞系的IC50值范围为17.65至84.55M。此外，33个化合物，包括类黄酮,酚酸，香豆素,有机酸,蒽醌,和木脂素，通过使用UPLC-QTOF-MS/MS对提取物进行化学分析发现。一些分子，比如波弗林和glabrol，可能用于抗菌目的，根据计算机模拟研究，而大豆苷元4'-硫酸盐显示出作为抗癌代谢产物的前景。
