Abstract：
BACKGROUND: Hemotropic mycoplasmas, hemoplasmas, are epi-erythrocytic parasitic bacteria that can be transmitted through blood transfusion.
OBJECTIVE: To study the prevalence of hemoplasma infection of potential feline blood donors and investigate the association between Hemoplasma spp. quantitative polymerase chain reaction (qPCR) positivity in blood units and selected variables.
METHODS: Seven thousand five hundred seventy-three blood units from 4121 privately-owned potential donor cats.
METHODS: Retrospective observational cross-sectional study. The Banco Sangue Animal (BSA)-Animal Blood Bank medical database was reviewed for all feline donations performed in 2022 in Portugal, Spain, and Belgium. Baseline characteristics and results of blood-borne pathogens screening tests were extracted from the medical records.
RESULTS: Two hundred twelve of 4034 Portuguese donor cats and 2 of 70 Spanish donor cats tested positive for Hemoplasma spp. qPCR in 2022 leading to an overall estimated prevalence of 5.2% (95% CI: 4.5%-5.9%) in potential blood donors. Using multivariable generalized estimation equation models, Hemoplasma spp. qPCR was more often positive among blood units issued from male cats (OR = 1.9, 95% CI: 1.4-2.6, P < .0001), units positive for FeLV (OR = 2.8, 95% CI: 1.4-5.6, P = .0023), and units collected in winter months (OR = 2.5, 95% CI: 1.7-3.6, P < .0001).
CONCLUSIONS: This study underscores the importance of Hemoplasma spp. and other relevant blood-borne pathogens screening at every donation. Implementing stringent screening protocols is crucial to mitigate the risk of hemoplasma transmission via blood transfusions, thereby safeguarding the health and welfare of cats receiving transfusions.
OBJECTIVE: To study the prevalence of hemoplasma infection of potential feline blood donors and investigate the association between Hemoplasma spp. quantitative polymerase chain reaction (qPCR) positivity in blood units and selected variables.
METHODS: Seven thousand five hundred seventy-three blood units from 4121 privately-owned potential donor cats.
METHODS: Retrospective observational cross-sectional study. The Banco Sangue Animal (BSA)-Animal Blood Bank medical database was reviewed for all feline donations performed in 2022 in Portugal, Spain, and Belgium. Baseline characteristics and results of blood-borne pathogens screening tests were extracted from the medical records.
RESULTS: Two hundred twelve of 4034 Portuguese donor cats and 2 of 70 Spanish donor cats tested positive for Hemoplasma spp. qPCR in 2022 leading to an overall estimated prevalence of 5.2% (95% CI: 4.5%-5.9%) in potential blood donors. Using multivariable generalized estimation equation models, Hemoplasma spp. qPCR was more often positive among blood units issued from male cats (OR = 1.9, 95% CI: 1.4-2.6, P < .0001), units positive for FeLV (OR = 2.8, 95% CI: 1.4-5.6, P = .0023), and units collected in winter months (OR = 2.5, 95% CI: 1.7-3.6, P < .0001).
CONCLUSIONS: This study underscores the importance of Hemoplasma spp. and other relevant blood-borne pathogens screening at every donation. Implementing stringent screening protocols is crucial to mitigate the risk of hemoplasma transmission via blood transfusions, thereby safeguarding the health and welfare of cats receiving transfusions.
摘要：
背景：嗜血支原体，血原虫,是可以通过输血传播的表皮红细胞寄生虫。
目的：研究潜在猫科动物献血者的血血浆感染情况，并探讨血血浆与血血浆的关系。定量聚合酶链反应（qPCR）阳性的血液单位和选定的变量。
方法：来自4121只私人拥有的潜在供体猫的七千五百七十三个血液单位。
方法：回顾性观察性横断面研究。对2022年在葡萄牙进行的所有猫科动物捐赠的BancoSangue动物（BSA）-动物血库医学数据库进行了审查，西班牙,和比利时。从病历中提取血源性病原体筛查试验的基线特征和结果。
结果：4034只葡萄牙供体猫中的212只和70只西班牙供体猫中的2只的血支原体检测呈阳性。2022年qPCR导致潜在献血者的总体估计患病率为5.2％（95％CI：4.5％-5.9％）。使用多变量广义估计方程模型，血支原体属。qPCR在雄性猫的血液单位中更常见（OR=1.9，95％CI：1.4-2.6，P<0.0001），单位为FeLV阳性（OR=2.8，95%CI：1.4-5.6，P=.0023），和冬季收集的单位（OR=2.5，95%CI：1.7-3.6，P<0.0001）。
结论：本研究强调了血浆支原体的重要性。和其他相关的血液传播病原体筛查在每次捐赠。实施严格的筛查方案对于降低通过输血传播血浆的风险至关重要。从而保障接受输血的猫的健康和福利。
目的：研究潜在猫科动物献血者的血血浆感染情况，并探讨血血浆与血血浆的关系。定量聚合酶链反应（qPCR）阳性的血液单位和选定的变量。
方法：来自4121只私人拥有的潜在供体猫的七千五百七十三个血液单位。
方法：回顾性观察性横断面研究。对2022年在葡萄牙进行的所有猫科动物捐赠的BancoSangue动物（BSA）-动物血库医学数据库进行了审查，西班牙,和比利时。从病历中提取血源性病原体筛查试验的基线特征和结果。
结果：4034只葡萄牙供体猫中的212只和70只西班牙供体猫中的2只的血支原体检测呈阳性。2022年qPCR导致潜在献血者的总体估计患病率为5.2％（95％CI：4.5％-5.9％）。使用多变量广义估计方程模型，血支原体属。qPCR在雄性猫的血液单位中更常见（OR=1.9，95％CI：1.4-2.6，P<0.0001），单位为FeLV阳性（OR=2.8，95%CI：1.4-5.6，P=.0023），和冬季收集的单位（OR=2.5，95%CI：1.7-3.6，P<0.0001）。
结论：本研究强调了血浆支原体的重要性。和其他相关的血液传播病原体筛查在每次捐赠。实施严格的筛查方案对于降低通过输血传播血浆的风险至关重要。从而保障接受输血的猫的健康和福利。
