Abstract：
Hydrogen peroxide (H2O2) is naturally produced by plant cells during normal development and serves as a messenger that regulates cell metabolism. Despite its importance, the relationship between hydrogen peroxide and the target of rapamycin (TOR) pathway, as well as its impact on cell division, has been poorly analyzed. In this study, we explore the interaction of H2O2 with TOR, a serine/threonine protein kinase that plays a central role in controlling cell growth, size, and metabolism in Arabidopsis thaliana. By applying two concentrations of H2O2 exogenously (0.5 and 1 mM), we could correlate developmental traits, such as primary root growth, lateral root formation, and fresh weight, with the expression of the cell cycle gene CYCB1;1, as well as TOR expression. When assessing the expression of the ribosome biogenesis-related gene RPS27B, an increase of 94.34% was noted following exposure to 1 mM H2O2 treatment. This increase was suppressed by the TOR inhibitor torin 2. The elimination of H2O2 accumulation with ascorbic acid (AA) resulted in decreased cell division as well as TOR expression. The potential molecular mechanisms associated with the effects of H2O2 on the cell cycle and TOR expression in roots are discussed in the context of the results.
摘要：
过氧化氢（H2O2）是植物细胞在正常发育过程中自然产生的，是调节细胞代谢的信使。尽管它很重要,过氧化氢与雷帕霉素靶蛋白(TOR)通路的关系,以及它对细胞分裂的影响，分析得很差。在这项研究中,我们探索H2O2与TOR的相互作用，一种丝氨酸/苏氨酸蛋白激酶，在控制细胞生长中起着核心作用，尺寸,和拟南芥的新陈代谢。通过外源施用两种浓度的H2O2（0.5和1mM），我们可以关联发育特征，如主根生长，侧根形成，和新鲜的重量，与细胞周期基因CYCB1的表达；1，以及TOR的表达。当评估核糖体生物发生相关基因RPS27B的表达时，暴露于1mMH2O2处理后，增加了94.34％。这种增加被TOR抑制剂Torin2抑制。用抗坏血酸（AA）消除H2O2积累导致细胞分裂和TOR表达减少。在结果的背景下讨论了与H2O2对根部细胞周期和TOR表达的影响相关的潜在分子机制。
