Abstract：
Cardiac myxoma is the most common primary cardiac tumor in adults. The histogenesis and cellular composition of myxoma are still unclear. This study aims to reveal the role of myxoma cell components and their gene expression in tumor development. We obtained single living cells by enzymatic digestion of tissues from 4 cases of surgically resected cardiac myxoma. Of course, there was 1 case of glandular myxoma and 3 cases of nonglandular myxoma. Then, 10× single-cell sequencing was performed. We identified 12 types and 11 types of cell populations in glandular myxoma and nonglandular myxoma, respectively. Heterogeneous epithelial cells are the main components of glandular myxoma. The similarities and differences in T cells in both glandular and nonglandular myxoma were analyzed by KEGG and GO. The most important finding was that there was active communication between T cells and epithelial cells. These results clarify the possible tissue occurrence and heterogeneity of cardiac myxoma and provide a theoretical basis and guidance for clinical diagnosis and treatment.
摘要：
心脏粘液瘤是成人最常见的原发性心脏肿瘤。粘液瘤的组织发生和细胞组成尚不清楚。本研究旨在揭示粘液瘤细胞成分及其基因表达在肿瘤发生发展中的作用。我们通过酶消化4例手术切除的心脏粘液瘤的组织获得了单个活细胞。当然,腺性粘液瘤1例，非腺性粘液瘤3例。然后，进行10X单细胞测序。我们在腺性粘液瘤和非腺性粘液瘤中确定了12种类型和11种类型的细胞群，分别。异源性上皮细胞是腺体粘液瘤的主要构成部门。通过KEGG和GO分析腺性和非腺性粘液瘤中T细胞的异同。最重要的发现是T细胞和上皮细胞之间存在活跃的交流。这些结果阐明了心脏黏液瘤可能的组织发生和异质性,为临床诊断和治疗提供了理论依据和指导。
