Abstract:
Curcumin is a natural lipophilic polyphenol that exhibits significant various biological properties such as antioxidant and anti-inflammatory properties following oral administration. However, its uses have shown limitations concerning aqueous solubility, bioavailability and biodegradability that could be improved by prolamin-based nanoparticle. In this study, curcumin was encapsulated into prolamin from sorghum (SOP) and wheat (WHP) and distilled spirit spent grain (DSSGP), which was obtained after microbial proteolysis of the former two cereal grains. All the three prolamins showed clear variation of protein profiles and microstructure as confirmed by electrophoresis analysis, disulfide bond determination and Fourier-transform infrared spectroscopy (FTIR). For curcumin-loaded nanospheres (NPs) fabrication, three prolamin-based NPs shared features of spherical shape, uniform particle size, and smooth surface. The average size ranged from 122 to 193 nm depending on the prolamin variety and curcumin loading. In the experiments in vitro, curcumin showed significantly improved UV/thermal stability. Furthermore, DSSGP was more resistant to enzymatic digestion in vitro, hence achieving the controlled release of curcumin in gastrointestinal tract. Collectively, the results indicated the improved bioavailability and biodegradability of curcumin encapsulated by DSSGP, which would be an innovative potential encapsulant for effective protection and targeted delivery of hydrophobic compounds.
摘要:
姜黄素是一种天然的亲脂性多酚,其在口服给药后表现出显著的各种生物学特性,例如抗氧化和抗炎特性。然而,它的用途显示出在水溶性方面的局限性,基于醇溶蛋白的纳米颗粒可以改善的生物利用度和生物降解性。在这项研究中,姜黄素被封装在高粱(SOP)和小麦(WHP)和蒸馏酒精废谷物(DSSGP)的醇溶蛋白中,这是前两种谷物的微生物蛋白水解后获得的。通过电泳分析证实,三种醇溶蛋白均显示出明显的蛋白质谱和微观结构变化,二硫键测定和傅里叶变换红外光谱(FTIR)。对于姜黄素负载的纳米球(NPs)制造,三个基于醇溶蛋白的NP共享球形特征,均匀的颗粒大小,和光滑的表面。平均尺寸范围为122至193nm,这取决于醇溶蛋白品种和姜黄素负载。在体外实验中,姜黄素显示出显著改善的UV/热稳定性。此外,DSSGP对体外酶消化的抗性更强,从而实现了姜黄素在胃肠道的控释。总的来说,结果表明,DSSGP封装的姜黄素的生物利用度和生物降解性得到了改善,这将是有效保护和靶向递送疏水性化合物的创新潜在包封剂。