Abstract：
Bovine Papillomaviruses (BPVs) constitute a diverse group within the Papillomaviridae family, playing a crucial role in bovine health and economic considerations. This study investigates the dynamics of vertical transmission of BPV in cattle, focusing on five cows and their reproductive tissues, as well as three gravid cows and their fetuses. DNA and RNA samples were extracted from the warts, fetal skin, placenta, uterus, ovary, and blood of cows, as well as the skin and blood of fetuses. Polymerase Chain Reaction (PCR) targeted BPV types 1-6 and 8-14, was assessed in both cows and fetuses. Additionally, Reverse Transcription PCR (RT-PCR) examined BPV-2 E5 oncogene expression in the skin and reproductive sites of mother cows and fetuses. Our findings unveil a rich diversity of BPV types, including BPV-2, 3, 9, 10, 12, and 14, present in both maternal and fetal tissues. Intriguingly, certain types, namely BPV-4, 6, 8, and 11, were exclusively identified in maternal tissues A higher diversity of BPVs was observed in cow warts, followed by cow blood, fetal blood, and fetal skin. Strikingly similar BPV types in gravid cow blood and fetuses suggest primary dissemination through the bloodstream and transmission via the placenta, though detected in lower numbers in cow uterus and ovary. Histopathological analysis revealed no abnormalities in the reproductive tissues despite the presence of BPV. However, in one bladder sample from a cow that did not consume bracken fern, urothelial neoplasia in situ was observed. The study extends beyond detection, exploring the expression of the BPV-2 E5 oncogene in fetal tissues, providing insights into potential cell implications. Comparative analyses with previous studies highlight the uniqueness of our investigation, encompassing a broader array of BPV types in the gravid cows and their fetuses. The findings not only establish a foundation for further investigations into the mechanisms of vertical transmission but also highlight the need for targeted interventions and surveillance strategies to mitigate potential health risks associated with specific BPV types.
摘要：
牛乳头瘤病毒（BPV）构成乳头瘤病毒科中的一个多样化群体，在牛健康和经济考虑中起着至关重要的作用。本研究调查了牛的BPV垂直传播动力学，专注于五头奶牛和它们的生殖组织，以及三头妊娠母牛和它们的胎儿。从疣中提取DNA和RNA样本，胎儿皮肤,胎盘,子宫,子房,和牛的血，还有胎儿的皮肤和血液.在奶牛和胎儿中评估了靶向BPV类型1-6和8-14的聚合酶链反应(PCR)。此外,逆转录PCR（RT-PCR）检测了BPV-2E5癌基因在母牛和胎儿皮肤和生殖部位的表达。我们的发现揭示了丰富多样的BPV类型，包括BPV-2、3、9、10、12和14，存在于母体和胎儿组织中。有趣的是，某些类型,即BPV-4、6、8和11在母体组织中被专门鉴定。在牛疣中观察到更高的BPV多样性，接着是牛血，胎儿血,和胎儿皮肤。妊娠牛血液和胎儿中的BPV类型非常相似，表明主要通过血液传播和通过胎盘传播，尽管在牛子宫和卵巢中检测到的数量较少。组织病理学分析显示，尽管存在BPV，但生殖组织中没有异常。然而,在一头没有食用蕨类植物的母牛的膀胱样本中，观察到原位尿路上皮瘤形成。这项研究超出了检测范围，探索BPV-2E5癌基因在胎儿组织中的表达，提供对潜在细胞影响的见解。与以往研究的比较分析强调了我们调查的独特性，在妊娠奶牛及其胎儿中涵盖了更广泛的BPV类型。这些发现不仅为进一步调查垂直传播机制奠定了基础，而且还强调了有针对性的干预措施和监测策略的必要性，以减轻与特定BPV类型相关的潜在健康风险。
