Abstract：
The utilization of surface plasmon resonance (SPR) sensors for real-time label-free molecular interaction analysis is already being employed in the fields of in vitro diagnostics and biomedicine. However, the widespread application of SPR technology is hindered by its limited detection throughput and high cost. To address this issue, this study introduces a novel multifunctional MetaSPR high-throughput microplate biosensor featuring 3D nanocups array structure, aiming to achieve high-throughput screening with a reduced cost and enhanced speed. Different types of MetaSPR sensors and analytical detection methods have been developed for accurate antibody subtype identification, epitope binding, affinity determination, antibody collocation, and quantitative detection， greatly promoting the screening and analysis of early-stage antibody drugs. The MetaSPR platform combined with nano-enhanced particles amplifies the detection signal and improves the detection sensitivity, making it more convenient, sensitive, and efficient than traditional ELISA. The findings demonstrate that the MetaSPR biosensor is a new practical technology detection platform that can improve the efficiency of biomolecular interaction studies with unlimited potential for new drug development.
摘要：
利用表面等离子体共振（SPR）传感器进行实时无标记分子相互作用分析已经在体外诊断和生物医学领域中得到应用。然而,SPR技术因其有限的检测通量和较高的成本而阻碍了其广泛应用。为了解决这个问题，我们的研究介绍了一种新型的多功能MetaSPR高通量微孔板生物传感器，具有3D纳米芯片，旨在以更低的成本和更高的速度实现高通量筛选。已经开发了不同类型的MetaSPR传感器和分析检测方法，用于准确的亚型识别，表位结合，亲和力测定，抗体搭配，等应用，极大地促进了早期抗体药物的筛选和分析。MetaSPR平台结合纳米增强粒子放大了检测信号,提高了检测灵敏度,使它更方便，敏感,并且比传统的ELISA高效。我们的发现表明，MetaSPR生物传感器是一种新的实用技术检测平台，可以提高生物分子相互作用研究的效率，具有无限的新药开发潜力。本文受版权保护。保留所有权利。
