PDF(Pubmed)
Abstract：
UNASSIGNED: Medical imaging modalities, such as magnetic resonance imaging (MRI), ultrasound, and fluorescence imaging, have gained widespread acceptance in clinical practice for tumor diagnosis. Each imaging modality has its own unique principles, advantages, and limitations, thus necessitating a multimodal approach for a comprehensive disease understanding of the disease process. To enhance diagnostic precision, physicians frequently integrate data from multiple imaging modalities, driving research advancements in multimodal imaging technology research.
UNASSIGNED: In this study, hematoporphyrin-poly (lactic acid) (HP-PLLA) polymer was prepared via ring-opening polymerization and thoroughly characterized using FT-IR, 1H-NMR, XRD, and TGA. HP-PLLA based nanoparticles encapsulating perfluoropentane (PFP) and salicylic acid were prepared via emulsion-solvent evaporation. Zeta potential and mean diameter were assessed using DLS and TEM. Biocompatibility was evaluated via cell migration, hemolysis, and cytotoxicity assays. Ultrasonic imaging was performed with a dedicated apparatus, while CEST MRI was conducted using a 7.0 T animal scanner.
UNASSIGNED: We designed and prepared a novel dual-mode nanoimaging probe SA/PFP@HP-PLLA NPs. PFP enhanced US imaging, while salicylic acid bolstered CEST imaging. With an average size of 74.43 ± 1.12 nm, a polydispersity index of 0.175 ± 0.015, and a surface zeta potential of -64.1 ± 2.11 mV. These NPs exhibit excellent biocompatibility and stability. Both in vitro and in vivo experiments confirmed the SA/PFP@HP-PLLA NP\'s ability to improve tumor characterization and diagnostic precision.
UNASSIGNED: The SA/PFP@HP-PLLA NPs demonstrate promising dual-modality imaging capabilities, indicating their potential for preclinical and clinical use as a contrast agent.
UNASSIGNED: In this study, hematoporphyrin-poly (lactic acid) (HP-PLLA) polymer was prepared via ring-opening polymerization and thoroughly characterized using FT-IR, 1H-NMR, XRD, and TGA. HP-PLLA based nanoparticles encapsulating perfluoropentane (PFP) and salicylic acid were prepared via emulsion-solvent evaporation. Zeta potential and mean diameter were assessed using DLS and TEM. Biocompatibility was evaluated via cell migration, hemolysis, and cytotoxicity assays. Ultrasonic imaging was performed with a dedicated apparatus, while CEST MRI was conducted using a 7.0 T animal scanner.
UNASSIGNED: We designed and prepared a novel dual-mode nanoimaging probe SA/PFP@HP-PLLA NPs. PFP enhanced US imaging, while salicylic acid bolstered CEST imaging. With an average size of 74.43 ± 1.12 nm, a polydispersity index of 0.175 ± 0.015, and a surface zeta potential of -64.1 ± 2.11 mV. These NPs exhibit excellent biocompatibility and stability. Both in vitro and in vivo experiments confirmed the SA/PFP@HP-PLLA NP\'s ability to improve tumor characterization and diagnostic precision.
UNASSIGNED: The SA/PFP@HP-PLLA NPs demonstrate promising dual-modality imaging capabilities, indicating their potential for preclinical and clinical use as a contrast agent.
摘要：
■医学成像模式，如磁共振成像（MRI），超声，和荧光成像，在肿瘤诊断的临床实践中获得了广泛的认可。每种成像模式都有自己独特的原则，优势,和限制，因此，需要一种多模式方法来全面了解疾病过程。为了提高诊断精度，医生经常整合来自多种成像模式的数据，推动多模态成像技术研究的进步。
■在这项研究中，通过开环聚合制备了血卟啉-聚乳酸（HP-PLLA）聚合物，并使用FT-IR进行了全面表征，1H-NMR,XRD,和TGA。通过乳液-溶剂蒸发制备包封全氟戊烷(PFP)和水杨酸的基于HP-PLLA的纳米颗粒。使用DLS和TEM评估ζ电位和平均直径。通过细胞迁移评估生物相容性，溶血,和细胞毒性测定。超声成像是用专用设备进行的，CESTMRI使用7.0T动物扫描仪进行。
■我们设计并制备了一种新型的双模纳米成像探针SA/PFP@HP-PLLANP。PFP增强US成像，而水杨酸支持CEST成像。平均尺寸为74.43±1.12nm，多分散指数为0.175±0.015,表面ζ电位为-64.1±2.11mV。这些NP表现出优异的生物相容性和稳定性。体外和体内实验均证实了SA/PFP@HP-PLLANP提高肿瘤特征和诊断精度的能力。
■SA/PFP@HP-PLLANP展示了有希望的双模态成像功能，表明它们作为造影剂的临床前和临床应用的潜力。
■在这项研究中，通过开环聚合制备了血卟啉-聚乳酸（HP-PLLA）聚合物，并使用FT-IR进行了全面表征，1H-NMR,XRD,和TGA。通过乳液-溶剂蒸发制备包封全氟戊烷(PFP)和水杨酸的基于HP-PLLA的纳米颗粒。使用DLS和TEM评估ζ电位和平均直径。通过细胞迁移评估生物相容性，溶血,和细胞毒性测定。超声成像是用专用设备进行的，CESTMRI使用7.0T动物扫描仪进行。
■我们设计并制备了一种新型的双模纳米成像探针SA/PFP@HP-PLLANP。PFP增强US成像，而水杨酸支持CEST成像。平均尺寸为74.43±1.12nm，多分散指数为0.175±0.015,表面ζ电位为-64.1±2.11mV。这些NP表现出优异的生物相容性和稳定性。体外和体内实验均证实了SA/PFP@HP-PLLANP提高肿瘤特征和诊断精度的能力。
■SA/PFP@HP-PLLANP展示了有希望的双模态成像功能，表明它们作为造影剂的临床前和临床应用的潜力。
