UNASSIGNED: In this study, hematoporphyrin-poly (lactic acid) (HP-PLLA) polymer was prepared via ring-opening polymerization and thoroughly characterized using FT-IR, 1H-NMR, XRD, and TGA. HP-PLLA based nanoparticles encapsulating perfluoropentane (PFP) and salicylic acid were prepared via emulsion-solvent evaporation. Zeta potential and mean diameter were assessed using DLS and TEM. Biocompatibility was evaluated via cell migration, hemolysis, and cytotoxicity assays. Ultrasonic imaging was performed with a dedicated apparatus, while CEST MRI was conducted using a 7.0 T animal scanner.
UNASSIGNED: We designed and prepared a novel dual-mode nanoimaging probe SA/PFP@HP-PLLA NPs. PFP enhanced US imaging, while salicylic acid bolstered CEST imaging. With an average size of 74.43 ± 1.12 nm, a polydispersity index of 0.175 ± 0.015, and a surface zeta potential of -64.1 ± 2.11 mV. These NPs exhibit excellent biocompatibility and stability. Both in vitro and in vivo experiments confirmed the SA/PFP@HP-PLLA NP\'s ability to improve tumor characterization and diagnostic precision.
UNASSIGNED: The SA/PFP@HP-PLLA NPs demonstrate promising dual-modality imaging capabilities, indicating their potential for preclinical and clinical use as a contrast agent.
■在这项研究中,通过开环聚合制备了血卟啉-聚乳酸(HP-PLLA)聚合物,并使用FT-IR进行了全面表征,1H-NMR,XRD,和TGA。通过乳液-溶剂蒸发制备包封全氟戊烷(PFP)和水杨酸的基于HP-PLLA的纳米颗粒。使用DLS和TEM评估ζ电位和平均直径。通过细胞迁移评估生物相容性,溶血,和细胞毒性测定。超声成像是用专用设备进行的,CESTMRI使用7.0T动物扫描仪进行。
■我们设计并制备了一种新型的双模纳米成像探针SA/PFP@HP-PLLANP。PFP增强US成像,而水杨酸支持CEST成像。平均尺寸为74.43±1.12nm,多分散指数为0.175±0.015,表面ζ电位为-64.1±2.11mV。这些NP表现出优异的生物相容性和稳定性。体外和体内实验均证实了SA/PFP@HP-PLLANP提高肿瘤特征和诊断精度的能力。
■SA/PFP@HP-PLLANP展示了有希望的双模态成像功能,表明它们作为造影剂的临床前和临床应用的潜力。