PDF(Pubmed)
Abstract：
The evidence on the role of diets in the production of short-chain fatty acids (SCFAs) was limited. The aim of this study was to assess the potential effects of high-fat high-fructose (HFHF), high-fat, and Western diets on the levels of SCFA. A research experiment employing a post-test-only control group design was carried out from January to April 2022. A total of 27 rats were randomly allocated to each study group. SCFA was measured two weeks after diet administration. Analysis of variance (ANOVA) test was used to analyze the differences among groups, and the effect estimate of each group was analyzed using post hoc Tukey. The concentrations of SCFAs post HFHF diets were recorded as follows: acetic acid at 54.60±10.58 mmol/g, propionic acid at 28.03±8.81 mmol/g, and butyric acid at 4.23±1.68 mmol/g. Following the high-fat diet, acetic acid measured 61.85±14.25 mmol/gr, propionic acid measured 25.19±5.55 mmol/gr, and butyric acid measured 6.10±2.93 mmol/gr. After the administration of Western diet, the levels of SCFA were 68.18±25.73, 29.69±12.76, and 7.48±5.51 mmol/g for acetic acid, propionic acid, and butyric acid, respectively. The level of butyric acid was significantly lower in HFHF diet group compared to the normal diet (mean difference (MD) 6.34; 95%CI: 0.61, 12.04; p=0.026). The levels of acetic acid (p=0.419) and propionic acid (p=0.316) were not statistically different among diet types (HFHF, high-fat, and Western diet). In conclusion, HFHF diet is associated with a lower level of butyric acid than the normal diet in a rat model.
摘要：
关于饮食在短链脂肪酸（SCFA）生产中的作用的证据有限。这项研究的目的是评估高脂肪高果糖（HFHF）的潜在影响，高脂肪,和西方饮食对SCFA水平的影响。2022年1月至4月进行了一项采用仅测试后对照组设计的研究实验。将总共27只大鼠随机分配到每个研究组。在饮食施用后两周测量SCFA。采用方差分析(ANOVA)检验分析组间差异,并使用事后Tukey分析每组的效果估计。HFHF饮食后SCFA的浓度记录如下：乙酸为54.60±10.58mmol/g，丙酸为28.03±8.81mmol/g，和丁酸为4.23±1.68mmol/g。在高脂肪饮食之后，测得乙酸为61.85±14.25mmol/gr，丙酸测量25.19±5.55mmol/gr，和丁酸测量为6.10±2.93mmol/gr。西方饮食管理后，乙酸的SCFA水平分别为68.18±25.73、29.69±12.76和7.48±5.51mmol/g，丙酸,和丁酸,分别。与正常饮食相比，HFHF饮食组的丁酸水平显着降低（平均差异（MD）6.34；95CI：0.61，12.04；p=0.026）。在饮食类型之间，乙酸（p=0.419）和丙酸（p=0.316）的水平没有统计学差异（HFHF，高脂肪,和西方饮食）。总之,在大鼠模型中,HFHF饮食与比正常饮食更低的丁酸水平相关。
