PDF(Pubmed)
Abstract：
Cryptosporidium spp., Giardia spp. and Enterocytozoon bieneusi are common zoonotic pathogens in humans and animals. Although rodents are important parts of the ecosystem and common hosts for these pathogens, little is known of the distribution, genetic diversity and zoonotic potential of these pathogens in wild rodents. A total of 442 fecal samples were collected from eleven wild rodent species in three provinces of China, and analyzed for these pathogens by PCR and DNA sequencing. The infection rates of Cryptosporidium spp., Giardia spp. and E. bieneusi were 19.9% (88/442), 19.8% (75/378) and 12.2% (54/442), respectively. Altogether, 23 known Cryptosporidium species/genotypes were identified and their distribution varied among different sampling locations or rodent species. Subtyping of the zoonotic Cryptosporidium species identified two novel subtype families XVe and XVf in C. viatorum, the subtype family XIIh and a novel subtype family XIIj in C. ubiquitum, and the subtype family IId in C. parvum. Three Giardia species were identified, including G. microti (n = 57), G. muris (n = 15) and G. duodenalis (n = 3), with G. duodenalis assemblages A and G identified in brown rats in urban areas of Guangdong. In addition, 13 E. bieneusi genotypes including eight known and five novel ones were identified, belonging to Groups 1, 2, 10, 14 and 15. Within nine genotypes in the zoonotic Group 1, common human-pathogenic genotypes D, Type IV, PigEbITS7 and Peru8 were detected only in brown rats and Lesser rice-field rats in urban areas of Guangdong. Apparent host adaptation and geographical differences were observed among Cryptosporidium spp., Giardia spp. and E. bieneusi genotypes in wild rodents in the present study. Furthermore, the zoonotic Cryptosporidium species and E. bieneusi genotypes commonly found here suggest a high zoonotic potential of these pathogens in wild rodents, especially in brown rats in urban areas. Hygiene and One Health measures should be implemented in urban streets and food stores to reduce the possible direct and indirect transmission of these rodent-related pathogens.
摘要：
隐孢子虫。,贾第虫。肠孢子虫是人类和动物常见的人畜共患病原体。尽管啮齿动物是生态系统的重要组成部分和这些病原体的常见宿主，对分布知之甚少，这些病原体在野生啮齿动物中的遗传多样性和人畜共患潜力。从中国三个省的11种野生啮齿动物中收集了442份粪便样本，并通过PCR和DNA测序分析这些病原体。隐孢子虫的感染率。,贾第虫。E.bieneusi为19.9%(88/442)，19.8%(75/378)和12.2%(54/442)，分别。总之,确定了23种已知的隐孢子虫物种/基因型，它们的分布在不同的采样位置或啮齿动物物种之间有所不同。人畜共患隐孢子虫的亚型鉴定出两个新的亚型家族XVe和XVf，泛素杆菌中的亚型家族XIIh和一个新的亚型家族XIIj，和C.parvum中的亚型家族IId。确定了三种贾第虫，包括G.microti(n=57)，G.muris（n=15）和G.daudenalis（n=3），在广东省市区的棕色大鼠中鉴定出十二指肠G.组合A和G。此外,鉴定出13种E.bieneusi基因型，包括8种已知基因型和5种新型基因型，属于第1、2、10、14和15组。在人畜共患组1中的9种基因型中，常见的人类致病性基因型D，IV型,仅在广东省城区的棕色大鼠和小稻田大鼠中检测到PigEbITS7和Peru8。在隐孢子虫中观察到明显的宿主适应和地理差异。,贾第虫。本研究中野生啮齿动物的E.bieneusi基因型。此外，这里常见的人畜共患隐孢子虫和E.bieneusi基因型表明，这些病原体在野生啮齿动物中具有很高的人畜共患潜力，尤其是城市地区的棕色老鼠。应在城市街道和食品商店实施卫生和一卫生措施，以减少这些与啮齿动物有关的病原体的可能直接和间接传播。
