PDF(Pubmed)
Abstract：
UNASSIGNED: In Kazakhstan, the study of Echinococcus infection among farm animals is crucial to monitor the invasion among livestock and map the data obtained. Unfortunately, there are only partial data on the study of Echinococcus among cattle\'s in Kazakhstan, which makes it difficult to conduct a comparative analysis of the epidemiological situation among livestock animals. The present study aimed to molecularly identify the species and haplotypes of the E. granulosus complex infecting cattle in Kazakhstan and investigate their genetic variation relative to mitochondrial (mt) targets.
UNASSIGNED: Individual cyst isolates (n = 700) were collected from infected cattle lungs and livers after slaughter from the slaughterhouse. Total DNA was extracted from the germinal layers of the cyst from each isolate. This DNA sequenced partial mt genes of cytochrome c oxidase 1 (450 bp) and NADH dehydrogenase 1 (1200 bp).
UNASSIGNED: We determined that all the sequences were detected as E. granulosus s.s., of which 69 (94.5%) samples belonged to G1, and only 4 (5.4%) samples belonged to the G3 genotype. After bioinformatic analysis, 38 haplotypes were identified.
UNASSIGNED: Our findings revealed that the G1 genotype of E. granulosus s.s. is the predominant cattle genotype in Kazakhstan. However, only one region showed the presence of two genotypes G1 and G3, in the sequence, which suggests that further research is needed to investigate the epidemiology of Echinococcus infection in cattle in Kazakhstan.
UNASSIGNED: Individual cyst isolates (n = 700) were collected from infected cattle lungs and livers after slaughter from the slaughterhouse. Total DNA was extracted from the germinal layers of the cyst from each isolate. This DNA sequenced partial mt genes of cytochrome c oxidase 1 (450 bp) and NADH dehydrogenase 1 (1200 bp).
UNASSIGNED: We determined that all the sequences were detected as E. granulosus s.s., of which 69 (94.5%) samples belonged to G1, and only 4 (5.4%) samples belonged to the G3 genotype. After bioinformatic analysis, 38 haplotypes were identified.
UNASSIGNED: Our findings revealed that the G1 genotype of E. granulosus s.s. is the predominant cattle genotype in Kazakhstan. However, only one region showed the presence of two genotypes G1 and G3, in the sequence, which suggests that further research is needed to investigate the epidemiology of Echinococcus infection in cattle in Kazakhstan.
摘要：
■在哈萨克斯坦，农场动物棘球蚴感染的研究对于监测牲畜的入侵和绘制获得的数据至关重要。不幸的是,哈萨克斯坦的牛中棘球蚴的研究只有部分数据，这使得很难对家畜动物之间的流行病学情况进行比较分析。本研究旨在分子鉴定哈萨克斯坦感染牛的E.granulosus复合体的物种和单倍型，并研究其相对于线粒体（mt）靶标的遗传变异。
■从屠宰场屠宰后，从感染的牛肺和肝脏中收集单个囊肿分离株（n=700）。从每个分离株的囊肿的生发层中提取总DNA。该DNA测序了细胞色素c氧化酶1（450bp）和NADH脱氢酶1（1200bp）的部分mt基因。
■我们确定所有序列均检测为E.granulosuss.s.，其中69份（94.5%）样本属于G1，只有4份（5.4%）样本属于G3基因型。经过生物信息学分析，鉴定出38个单倍型。
■我们的发现表明，S.S.的G1基因型是哈萨克斯坦的主要牛基因型。然而,序列中只有一个区域显示出两种基因型G1和G3的存在，这表明需要进一步的研究来调查哈萨克斯坦牛的棘球蚴感染的流行病学。
■从屠宰场屠宰后，从感染的牛肺和肝脏中收集单个囊肿分离株（n=700）。从每个分离株的囊肿的生发层中提取总DNA。该DNA测序了细胞色素c氧化酶1（450bp）和NADH脱氢酶1（1200bp）的部分mt基因。
■我们确定所有序列均检测为E.granulosuss.s.，其中69份（94.5%）样本属于G1，只有4份（5.4%）样本属于G3基因型。经过生物信息学分析，鉴定出38个单倍型。
■我们的发现表明，S.S.的G1基因型是哈萨克斯坦的主要牛基因型。然而,序列中只有一个区域显示出两种基因型G1和G3的存在，这表明需要进一步的研究来调查哈萨克斯坦牛的棘球蚴感染的流行病学。
