关键词: 16S‐rDNA SCFA biomarker faecal microbiome semen swine

Mesh : Animals Male Feces / microbiology Sperm Motility Acinetobacter / isolation & purification Housing, Animal Gastrointestinal Microbiome Sus scrofa Fatty Acids, Volatile / analysis RNA, Ribosomal, 16S / analysis Floors and Floorcoverings Swine

来  源:   DOI:10.1111/rda.14616

Abstract:
The swine gastrointestinal tract contains a great variety of microbes, forming a complex and dynamic ecosystem. Various internal and external factors (e.g. age, breed and diet) may influence its composition. This study aimed to investigate the gut microbial diversity of German Piétrain boars housed on different deep-litter bedding materials (regional wood shavings, linen, hemp, spelt husks, and wood shavings) via 16S-rDNA sequencing. Additionally, short-chain fatty acids were analysed using gas chromatography. Fresh faecal samples (n = 80) from 40 Piétrain boars were collected twice during the trial. Although it can be assumed that boars ingest bedding orally, no differences in the microbiome composition could be found. The main phyla were Firmicutes and Bacteroides. Acinetobacter was identified as a biomarker for sperm quality differences (total sperm motility) in breeding boars.
摘要:
猪的胃肠道含有各种各样的微生物,形成一个复杂而动态的生态系统。各种内部和外部因素(如年龄、品种和饮食)可能会影响其组成。这项研究旨在调查饲养在不同深砂垫料上的德国Pietrain公猪的肠道微生物多样性(区域刨花,亚麻,大麻,拼写外壳,和刨花)通过16S-rDNA测序。此外,短链脂肪酸使用气相色谱分析。在试验过程中,从40只Pietrainboars中收集了两次新鲜的粪便样本(n=80)。尽管可以假设公猪口服了被褥,微生物组组成没有差异。主要的门是Firmicutes和拟杆菌属。不动杆菌被鉴定为育种公猪中精子质量差异(总精子运动性)的生物标志物。
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