Abstract：
Abdominal aortic aneurysm (AAA) is a vascular disease characterized by progressive dilation of the abdominal aorta. Previous studies have suggested that dietary components are closely associated with AAA. Among those dietary components, eicosapentaenoic acid (EPA) is considered to have suppressive effects on AAA. In the AAA wall of AAA model animals bred under EPA-rich condition, the distribution of EPA-containing phosphatidylcholine (EPA-PC) has been reported to be similar to that of the markers of mesenchymal stem cells (MSCs) and M2 macrophages. These data suggest that the suppressive effects of EPA on AAA are related to preferential distribution of specific cells in the aortic wall. However, the distribution of EPA-PC in the AAA wall of AAA model animals fed a diet containing small amounts of EPA, which has not been reported to inhibit AAA, has not yet been explored. In the present study, we visualized the distribution of EPA-PCs in the AAA wall of AAA model animals fed a diet containing small amounts of EPA (1.5% EPA in the fatty acid composition) to elucidate the vasoprotective effects of EPA. Positive areas for markers of MSCs were significantly higher in the region where EPA-PC was abundant compared to the regions where EPA-PC was weakly detected, but not for markers of M2 macrophages, matrix metalloproteinase (MMP)-2, and MMP-9. The distribution of MSC markers was similar to that of EPA-PC but not that of M2 macrophages and MMPs. These data suggest preferential incorporation of EPA into MSCs under the conditions used in this study. The incorporation of EPA into certain cells may differ according to dietary conditions, which affect the development of AAA.
摘要：
腹主动脉瘤（AAA）是一种以腹主动脉进行性扩张为特征的血管疾病。先前的研究表明，膳食成分与AAA密切相关。在这些饮食成分中，二十碳五烯酸（EPA）被认为对AAA具有抑制作用。在富含EPA条件下饲养的AAA模型动物的AAA壁中，据报道,含有EPA的磷脂酰胆碱(EPA-PC)的分布与间充质干细胞(MSC)和M2巨噬细胞的标志物相似.这些数据表明，EPA对AAA的抑制作用与特定细胞在主动脉壁中的优先分布有关。然而,饲喂含有少量EPA的AAA模型动物的AAA壁中EPA-PC的分布，尚未报告抑制AAA，尚未探索。在本研究中,我们观察了饲喂含少量EPA(脂肪酸组成中EPA含量为1.5%)的AAA模型动物的AAA壁中EPA-PCs的分布,以阐明EPA的血管保护作用.与EPA-PC检测较弱的区域相比，EPA-PC丰富的区域中MSC标志物的阳性区域显着更高。但不是M2巨噬细胞的标记，基质金属蛋白酶(MMP)-2和MMP-9。MSC标记的分布与EPA-PC相似，但与M2巨噬细胞和MMP相似。这些数据表明在本研究中使用的条件下EPA优先掺入MSC中。EPA在某些细胞中的掺入可能因饮食条件而异，影响AAA的发展。
