Abstract：
Triphenyl phosphate (TPhP) and transition metal elements have been ubiquitously detected in the atmosphere, which can participate in atmospheric chemical reactions and induce damage to human health. Currently the understanding of TPhP degradation, transformation and cytotoxicity on atmospheric particles surface are still limited. Therefore, this study used laboratory simulation methods to investigate the influence of irradiation time, transition metal salts, relative humidity (RH) to TPhP degradation, transformation and relative cytotoxicity. TPhP was coated on particle surfaces of four transition metal salts (MnSO4, CuSO4, FeSO4 and Fe2(SO4)3) in the experiment. Within 12 h irradiation, the significant TPhP photodegradation can be observed on all particles surface. Among these influence factors, the irradiation and RH were the crucial aspects to TPhP degradation, which primarily affect the OH concentration in the atmosphere. The transition metal elements only exhibited slightly catalytic effect to TPhP degradation. The mechanism study indicated that the major degradation products of TPhP are diphenyl hydrogen phosphate (DPhP) and OH-DPhP, which originated from the phenoxy bond cleavage and hydroxylation of TPhP induced by OH. As for the cytotoxicity to A549 cells, all the transition metal particles coated with TPhP can cause cellular injury, which was chiefly induced by the transition metal salt. The possible cytotoxicity mechanism of these particles to A549 cells can be attributed to the excessive reactive oxygen species (ROS) production. This study may provide a further understanding of TPhP degradation and related cytotoxicity with the coexistent transition metal salts in the atmosphere.
摘要：
磷酸三苯酯(TPhP)和过渡金属元素在大气中被广泛检测到,参与大气化学反应,对人体健康造成损害。目前对TPhP降解的认识,大气颗粒物表面的转化和细胞毒性仍然有限。因此,本研究采用实验室模拟方法来研究辐照时间的影响，过渡金属盐,相对湿度（RH）到TPhP降解，转化和相对细胞毒性。在实验中，将TPhP涂覆在四种过渡金属盐（MnSO4，CuSO4，FeSO4和Fe2（SO4）3）的颗粒表面上。照射12h内,在所有颗粒表面都可以观察到显著的TPhP光降解。在这些影响因素中,辐照和RH是TPhP降解的关键方面，主要影响大气中的OH浓度。过渡金属元素对TPhP降解仅表现出轻微的催化作用。机理研究表明,TPhP的主要降解产物为磷酸氢二苯酯(DPhP)和OH-DPhP,它起源于OH诱导的TPhP的苯氧基键断裂和羟基化。至于对A549细胞的细胞毒性，所有涂有TPhP的过渡金属颗粒都会引起细胞损伤，主要由过渡金属盐诱导。这些颗粒对A549细胞的可能的细胞毒性机制可归因于过量的活性氧(ROS)产生。这项研究可能会提供对大气中共存过渡金属盐的TPhP降解和相关细胞毒性的进一步了解。
