Abstract：
6-mercaptopurine (6-MP) is widely used in the treatment of many diseases, but exhibits some serious side effects due to its toxicity. Therefore, it is important and imperative to effectively control and monitoring concentration of 6-MP. Herein, we designed a smartphone-assisted colorimetric sensing platform for 6-MP detection, based on an excellent β-cyclodextrin modified MnO2 nanosheets (β-CD@MnO2 NNS) mediated oxidase-like activity. β-CD@MnO2 NNS can directly oxidizes 3,3\',5,5\'-tetramethylbenzidine (TMB) into oxidized TMB with color changes, yielding more than 3-fold higher oxidase-like catalytic activity compared with individual MnO2 NNS. After adding 6-MP, β-CD@MnO2 NNS can be reduced to Mn2+ and lose their oxidase-like properties, resulting in a color and absorbance change for sensitive and selectivity detection of 6-MP. Meanwhile, the smartphone-based color recognition application can intuitively and simply measure the concentration of 6-MP. The limits of detection UV-vis instrument and smartphone were 0.35 μM and 0.86 μM, respectively. This method has also been successfully applied to the detection of real samples. Finally, this study provides a new promising platform for detection of 6-MP and is expected to be used in application of pharmaceutical analysis and biomedicine.
摘要：
6-巯基嘌呤(6-MP)广泛应用于多种疾病的治疗,但由于其毒性而表现出一些严重的副作用。因此,有效控制和监测6-MP的浓度是非常重要和必要的。在这里，我们设计了一个用于6-MP检测的智能手机辅助比色传感平台，基于优异的β-环糊精修饰的MnO2纳米片（β-CD@MnO2NNS）介导的类氧化酶活性。β-CD@MnO2NNS可以直接氧化3,3'，5,5'-四甲基联苯胺（TMB）变成氧化的TMB，颜色变化，与单个MnO2NNS相比，产生3倍以上的氧化酶样催化活性。添加6-MP后，β-CD@MnO2NNS可还原成Mn2+并失去类氧化酶性质,导致颜色和吸光度变化，以灵敏和选择性检测6-MP。同时,基于智能手机的颜色识别应用程序可以直观，简单地测量6-MP的浓度。UV-vis仪器和智能手机的检测限分别为0.35μM和0.86μM，分别。该方法也已成功应用于实际样品的检测。最后,本研究为6-MP的检测提供了一个新的有前景的平台，有望在药物分析和生物医学中应用。
