Abstract：
Regular monitoring of Norovirus presence in environmental and food samples is crucial due to its high transmission rates and outbreak potential. For detecting Norovirus GI, reverse transcription qPCR method is commonly used, but its sensitivity can be affected by assay performance. This study shows significantly reduced assay performance in digital PCR or qPCR when using primers targeting Norovirus GI genome 5291-5319 (NC_001959), located on the hairpin of the predicted RNA structure. It is highly recommended to avoid this region in commercial kit development or diagnosis to minimizing potential risk of false negatives.
摘要：
定期监测诺如病毒在环境和食物样本中的存在至关重要，因为它的高传播率和爆发潜力。为了检测诺如病毒GI，逆转录qPCR方法是常用的,但其灵敏度会受到检测性能的影响。这项研究显示，当使用针对诺如病毒GI基因组5291-5319（NC_001959）的引物时，数字PCR或qPCR的检测性能显着降低，位于预测的RNA结构的发夹上。强烈建议在商业试剂盒开发或诊断中避免该区域，以最大程度地减少假阴性的潜在风险。
