Abstract：
This study evaluated the regulatory effects of Astragalus membranaceus polysaccharides (AMP) on lipid metabolism disorders induced by a high-fat diet (HFD) in spotted sea bass (Lateolabrax maculatus). Compared with the normal diets (10 % lipids), diets containing 15 % lipid levels were used as the high-fat diet (HFD). Three levels of the AMP (0.06 %, 0.08 %, 0.10 %) were added in the HFD and used as experimental diets. A total of 375 spotted sea bass (average weight 3.00 ± 0.01 g) were divided into 15 tanks and deemed as 5 groups, with each tank containing 25 fish. Fish in each group were fed with different diets for 56 days. After feeding, the HFD induced lipid metabolism disorders in fish, as evidenced by elevated serum lipids, malonaldehyde levels, and more severe liver damage. The AMP alleviated the HFD-induced liver damage, as evidenced by the reduced severity of liver histological lesions and malonaldehyde levels. The low-density lipoprotein cholesterol was reduced, and the expression of FAS and PPAR-α were down and up-regulated, respectively. However, the AMP had a limited ability to affect the serum lipids and abdominal fat percentage. These results reveal the potential of the AMP used in aquaculture to regulate lipid metabolism disorders induced by the HFD.
摘要：
本研究评估了黄芪多糖（AMP）对高脂饮食（HFD）引起的斑点鲈鱼（Lateolabraxmaculatus）脂质代谢紊乱的调节作用。与正常饮食（10％脂质）相比，使用含有15%脂质水平的饮食作为高脂肪饮食(HFD).三个水平的AMP（0.06％，0.08%,在HFD中添加0.10％）并用作实验饮食。总共375个斑点鲈鱼（平均重量3.00±0.01g）被分为15个坦克，并被视为5组，每个鱼缸里有25条鱼.每组鱼用不同的饮食喂养56天。喂食后，HFD诱导的鱼类脂质代谢紊乱，正如血清脂质升高所证明的那样，丙二醛水平，和更严重的肝损伤。AMP减轻了HFD引起的肝损伤，肝脏组织学病变的严重程度和丙二醛水平的降低证明了这一点。低密度脂蛋白胆固醇降低，FAS和PPAR-α的表达下调和上调，分别。然而,AMP对血脂和腹部脂肪百分比的影响能力有限。这些结果揭示了在水产养殖中使用的AMP调节由HFD诱导的脂质代谢紊乱的潜力。
