关键词: behavior cognitive neuroscience neuroscience

Mesh : Animals Globus Pallidus / diagnostic imaging physiology Wakefulness / physiology Behavior, Animal / physiology Stereotaxic Techniques Macaca mulatta Neurons / physiology cytology Magnetic Resonance Imaging / methods

来  源:   DOI:10.1016/j.xpro.2024.103081   PDF(Pubmed)

Abstract:
Extracellular recordings in behaving animals are useful for establishing associations between neuronal activity and behavior. Here, we describe how to record in the external globus pallidus (GPe) of monkeys engaged in a behavioral task. We detail the stereotaxic surgery for chamber and head-holder implantation, the post-operative MRI scan to ascertain the GPe coordinates and validate the position of the chamber, and the data collection. This protocol makes it possible to examine the electrophysiological features of GPe neurons in behaving monkeys. For complete details on the use and execution of this protocol, please refer to Katabi et al.1.
摘要:
行为动物的细胞外记录可用于建立神经元活动和行为之间的关联。这里,我们描述了如何记录参与行为任务的猴子的外部苍白球(GPe)。我们详细介绍了腔室和头部支架植入的立体定位手术,术后MRI扫描以确定GPe坐标并验证腔室的位置,和数据收集。该协议使得可以检查行为猴子中GPe神经元的电生理特征。有关此协议的使用和执行的完整详细信息,PleaserefertoKatabietal.1.
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