Abstract：
The construction of gating system in artificial channels is a cutting-edge research direction in understanding biological process and application sensing. Here, by mimicking the gating system, we report a device that easily synthesized single-glass micropipettes functionalized by three-dimensional (3D) DNA network, which triggers the gating mechanism for the detection of biomolecules. Based on this strategy, the gating mechanism shows that single-glass micropipette assembled 3D DNA network is in the \"OFF\" state, and after collapsing in the presence of ATP, they are in the \"ON\" state, at which point they exhibit asymmetric response times. In the \"ON\" process of the gating mechanism, the ascorbic acid phosphate (AAP) can be encapsulated by a 3D DNA network and released in the presence of adenosine triphosphate (ATP), which initiates a catalyzed cascade reaction under the influence of alkaline phosphatase (ALP). Ultimately, the detection of ALP can be responded to form the fluorescence signal generated by terephthalic acid that has captured hydroxyl radicals, which has a detection range of 0-250 mU/mL and a limit of detection of 50 mU/mL. This work provides a brand-new way and application direction for research of gating mechanism.
摘要：
在人工通道中构建浇注系统是理解生物过程和应用传感的前沿研究方向。这里,通过模仿浇注系统，我们报告了一种易于合成的单玻璃微量移液管的装置，该装置通过三维（3D）DNA网络功能化，这触发了生物分子检测的门控机制。基于这一战略,门控机制表明，单玻璃微量移液器组装的3DDNA网络处于“关闭”状态，在ATP存在下崩溃后，它们处于“ON”状态，在这一点上，它们表现出不对称的响应时间。在门控机构的“ON”过程中，抗坏血酸磷酸盐（AAP）可以被3DDNA网络封装，并在三磷酸腺苷（ATP）的存在下释放，在碱性磷酸酶（ALP）的影响下引发催化的级联反应。最终,ALP的检测可以响应形成由对苯二甲酸产生的捕获羟基自由基的荧光信号,其检测范围为0-250mU/mL,检测限为50mU/mL。这项工作为门控机构的研究提供了全新的途径和应用方向。
