Abstract：
Collagen is a complex, large protein molecule that presents a challenge in delivering it to the skin due to its size and intricate structure. However, conventional collagen delivery methods are either invasive or may affect the protein\'s structural integrity. This study introduces a novel approach involving the encapsulation of collagen monomers within zwitterionic nanoliposomes, termed Lip-Cols, and the controlled formation of collagen fibrils through electric fields (EF) stimulation. The results reveal the self-assembly process of Lip-Cols through electroporation and a pH gradient change uniquely triggered by EF, leading to the alignment and aggregation of Lip-Cols on the electrode interface. Notably, Lip-Cols exhibit the capability to direct the orientation of collagen fibrils within human dermal fibroblasts. In conjunction with EF, Lip-Cols can deliver collagen into the dermal layer and increase the collagen amount in the skin. The findings provide novel insights into the directed formation of collagen fibrils via electrical stimulation and the potential of Lip-Cols as a non-invasive drug delivery system for anti-aging applications.
摘要：
胶原蛋白是一种复杂的，大的蛋白质分子，由于其大小和复杂的结构，在将其传递到皮肤上提出了挑战。然而,传统的胶原蛋白递送方法要么是侵入性的，要么可能影响蛋白质的结构完整性。在这项研究中,我们介绍了一种新的方法，涉及将胶原蛋白单体封装在两性离子纳米脂质体中，称为Lip-Cos，和通过电场（EF）刺激控制胶原纤维的形成。我们的结果揭示了Lip-Cos通过电穿孔的自组装过程和EF独特触发的pH梯度变化，导致Lip-Cos在电极界面上的排列和聚集。值得注意的是,Lip-Cos具有指导人真皮成纤维细胞内胶原纤维取向的能力。与EF一起，Lip-Cos可以将胶原蛋白递送到真皮层中并增加皮肤中的胶原蛋白量。我们的发现为通过电刺激定向形成胶原纤维提供了新的见解，以及Lip-Cos作为抗衰老应用的非侵入性药物递送系统的潜力。本文受版权保护。保留所有权利。
