PDF(Pubmed)
Abstract：
Embryo rescue is a vital technique in cucurbit breeding and propagation, addressing challenges such as embryo abortion, poor seed viability, and incompatibility barriers. This method involves the excision of immature embryos from seeds followed by their in vitro culture on a nutrient medium, providing an environment conducive to their growth and development. In cucurbits, embryo rescue has been extensively utilized to overcome barriers to hybridization, enabling the production of interspecific and intergeneric hybrids with desired traits. Various factors, including genotype, developmental stage of embryos, and culture conditions, influence the success of embryo rescue in cucurbits. Optimal nutrient formulations, growth regulators, and culture techniques are critical for promoting embryo germination, shoot elongation, and subsequent plantlet establishment. Additionally, embryo rescue facilitates the recovery of valuable genetic material from wild and exotic cucurbit species, expanding genetic diversity and developing novel cultivars with improved traits such as disease resistance, yield, and quality. This review highlights the principles, applications, and advancements in embryo rescue technology in cucurbits, emphasizing its significance in cucurbit breeding programs and crop improvement efforts.
摘要：
胚胎拯救是葫芦育种和繁殖的重要技术。解决胚胎流产等挑战，种子活力差，和不相容的障碍。该方法涉及从种子中切除未成熟胚，然后在营养培养基上进行体外培养，提供有利于他们成长和发展的环境。在葫芦里,胚胎拯救已被广泛用于克服杂交障碍，能够产生具有所需性状的种间和属间杂种。各种因素,包括基因型，胚胎的发育阶段，和文化条件，影响南瓜胚胎抢救的成功。最佳营养配方，增长调节剂，培养技术对于促进胚胎萌发至关重要，枝条伸长率,以及随后的植株建立。此外,胚胎拯救有助于从野生和外来葫芦物种中恢复有价值的遗传物质，扩大遗传多样性，开发抗病性等性状得到改善的新品种，产量，和质量。这篇评论强调了原则，应用程序,以及南瓜胚胎拯救技术的进步，强调其在葫芦育种计划和作物改良工作中的重要性。
