PDF(Pubmed)
Abstract：
Medicinal plant tissue cultures are potential sources of bioactive compounds. In this study, we report the chemical characterization of the callus cultures of three medicinal Tilia spp. (Tilia cordata, Tilia vulgaris and Tilia tomentosa), along with the comparison to bracts and flowers of the same species. Our aim was to show that calli of Tilia spp. are good alternatives to the calli of T. americana for the production of polyphenols and are better sources of a subset of polyphenolic metabolites, compared to the original organs. Calli were initiated from young bracts and grown on woody plant medium containing 1 mg L-1 2,4-D and 0.1 mg L-1 BAP. For chemical characterization, a quality-controlled untargeted metabolomics approach and the quantification of several bioactive compounds was performed with the use of LC-ESI-MS/MS. While bracts and flowers contained flavonoid glycosides (astragalin, isoquercitrin) as major polyphenols, calli of all species contained catechins, coumarins (fraxin, esculin and scopoletin) and flavane aglyca. T. tomentosa calli contained 5397 µg g DW-1 catechin, 201 µg g DW-1 esculin, 218 µg g DW-1 taxifolin and 273 µg g DW-1 eriodictyol, while calli from other species contained lower amounts. T. cordata and T. tomentosa flowers were rich in isoquercitrin, containing 8134 and 6385 µg g DW-1, respectively. The currently tested species contained many of the bioactive metabolites described from T. americana. The production of catechin was shown to be comparable to the most efficient tissue cultures reported. Flowers and bracts contained flavonoid glycosides, including tiliroside, resembling bioactive fractions of T. americana. In addition, untargeted metabolomics has shown fingerprint-like differences among species, highlighting possible chemotaxonomic and quality control applications, especially for bracts.
摘要：
药用植物组织培养物是生物活性化合物的潜在来源。在这项研究中,我们报告了三种药用Tiliaspp的愈伤组织培养物的化学特征。(Tiliacordata,Tiliavulgaris和Tiliatomentosa)，以及与相同物种的苞片和花朵的比较。我们的目的是显示Tiliaspp的愈伤组织。是用于生产多酚的美洲T.americana愈伤组织的良好替代品，并且是多酚代谢物子集的更好来源，与原始器官相比。愈伤组织是从年轻的片开始的，并在含有1mgL-12,4-D和0.1mgL-1BAP的木本植物培养基上生长。对于化学表征，使用LC-ESI-MS/MS进行了质量控制的非靶向代谢组学方法和几种生物活性化合物的定量。而苞片和花含有类黄酮苷（黄芪，异槲皮苷)作为主要的多酚，所有物种的愈伤组织都含有儿茶素，香豆素(fraxin,七叶皂甙和四苯乙素）和黄烷aglyca。T.tomentosa愈伤组织含有5397µgDW-1儿茶素，201µgDW-1esculin,218µgDW-1紫杉酯和273µgDW-1紫杉醇，而来自其他物种的愈伤组织含量较低。紫花苜蓿和毛花富含异槲皮苷，分别含有8134和6385µgDW-1。当前测试的物种含有许多来自美洲T.americana的生物活性代谢物。显示儿茶素的产生与报道的最有效的组织培养物相当。花和苞片含有类黄酮苷，包括利洛苷,类似于美洲T.americana的生物活性部分。此外,非靶向代谢组学已经显示出物种之间的指纹样差异，强调可能的化学分类学和质量控制应用，尤其是对苞片。
