PDF(Pubmed)
Abstract：
The ancient Chinese medical book \"Compendium of Materia Medica\" records that pears can relieve symptoms of respiratory-related diseases. Previous research has shown that pear Pyrus Pyrifolia (Burm.f.) Nakai has antioxidant and anti-inflammatory properties. However, the anti-inflammatory, antioxidant, and anti-photoaging protective effects of Pyrus pyrifolia (Burm.f.) Nakai seed components have not been studied. Ultraviolet light (UV) causes skin inflammation, damages the skin barrier, and is an important cause of skin photoaging. Therefore, UV light with a wavelength of 365 nm was used to irradiate HaCaT and mice. Western blot, real-time quantitative polymerase chain reaction, and fluorescence imaging system were used to explore its anti-UVA mechanism. Dialysis membrane and nuclear magnetic resonance were used for the chemical constituent analysis of pear seed water extract (PSWE). We found that PSWE can significantly reduce UVA-induced skin cell death and mitogen-activated protein kinase phosphorylation and can inhibit the mRNA expression of UVA-induced cytokines (including IL-1β, IL-6, and TNF-α). In addition, PSWE can also reduce the generation of oxidative stress within skin cells. In vivo experimental studies found that PSWE pretreatment effectively reduced transepidermal water loss, inflammation, redness, and dryness in hairless mice. The molecular weight of the active part of pear water extract is approximately 384. Based on the above results, we first found that pear seeds can effectively inhibit oxidative stress and damage caused by UVA. It is a natural extract with antioxidant properties and anti-aging activity that protects skin cells and strengthens the skin barrier.
摘要：
中国古代医书《本草纲目》记载梨可以缓解呼吸道相关疾病的症状。先前的研究表明，梨梨Pyrifolia（Burm.f.）Nakai具有抗氧化和抗炎特性。然而,抗炎，抗氧化剂，以及Pyruspyrifolia（Burm.f.）Nakai种子成分的抗光老化保护作用尚未研究。紫外线（UV）引起皮肤炎症，破坏皮肤屏障，是皮肤光老化的重要原因。因此,使用波长为365nm的UV光照射HaCaT和小鼠。蛋白质印迹,实时定量聚合酶链反应，并利用荧光成像系统探讨其抗UVA的作用机制。采用透析膜和核磁共振技术对梨子水提取物(PSWE)进行化学成分分析。我们发现PSWE可以显着降低UVA诱导的皮肤细胞死亡和丝裂原活化蛋白激酶的磷酸化，并且可以抑制UVA诱导的细胞因子（包括IL-1β，IL-6和TNF-α)。此外,PSWE还可以减少皮肤细胞内氧化应激的产生。体内实验研究发现，PSWE预处理有效减少了经表皮水分流失，炎症,发红,无毛小鼠的干燥。梨水提取物的活性部分的分子量约为384。基于以上结果,我们首先发现梨种子能有效抑制UVA引起的氧化应激和损伤。它是一种具有抗氧化性能和抗衰老活性的天然提取物，可保护皮肤细胞并增强皮肤屏障。
