PDF(Pubmed)
Abstract：
Lumpy skin disease is one of the fast-spreading viral diseases of cattle and buffalo that can potentially cause severe economic impact. Lesotho experienced LSD for the first time in 1947 and episodes of outbreaks occurred throughout the decades. In this study, eighteen specimens were collected from LSD-clinically diseased cattle between 2020 and 2022 from Mafeteng, Leribe, Maseru, Berea, and Mohales\' Hoek districts of Lesotho. A total of 11 DNA samples were analyzed by PCR and sequencing of the extracellular enveloped virus (EEV) glycoprotein, G-protein-coupled chemokine receptor (GPCR), 30 kDa RNA polymerase subunit (RPO30), and B22R genes. All nucleotide sequences of the above-mentioned genes confirmed that the PCR amplicons of clinical samples are truly LSDV, as they were identical to respective LSDV isolates on the NCBI GenBank. Two of the elevem samples were further characterized by whole-genome sequencing. The analysis, based on both CaPV marker genes and complete genome sequences, revealed that the LSDV isolates from Lesotho cluster with the NW-like LSDVs, which includes the commonly circulating LSDV field isolates from Africa, the Middle East, the Balkans, Turkey, and Eastern Europe.
摘要：
结块皮肤病是牛和水牛快速传播的病毒性疾病之一，可能会造成严重的经济影响。莱索托在1947年首次经历了LSD，几十年来爆发了疫情。在这项研究中,在2020年至2022年之间，从Mafeteng的LSD临床病牛中收集了18个标本，莱里贝,Maseru,Berea,和莱索托的Mohales\'Hoek区。通过PCR和测序分析了总共11个DNA样品的细胞外包膜病毒（EEV）糖蛋白，G蛋白偶联趋化因子受体(GPCR),30kDaRNA聚合酶亚基(RPO30),和B22R基因。上述基因的所有核苷酸序列证实临床样品的PCR扩增子是真正的LSDV,因为它们与NCBIGenBank上的LSDV分离株相同。通过全基因组测序进一步表征两个elevem样品。分析,基于CaPV标记基因和完整的基因组序列，揭示了LSDV与类似NW的LSDV从莱索托集群中分离出来，其中包括来自非洲的常见的LSDV现场分离株，中东,巴尔干半岛,土耳其,和东欧。
