PDF(Pubmed)
Abstract：
Our current understanding of HSV latency is based on a variety of clinical observations, and in vivo, ex vivo, and in vitro model systems, each with unique advantages and drawbacks. The criteria for authentically modeling HSV latency include the ability to easily manipulate host genetics and biological pathways, as well as mimicking the immune response and viral pathogenesis in human infections. Although realistically modeling HSV latency is necessary when choosing a model, the cost, time requirement, ethical constraints, and reagent availability are also equally important. Presently, there remains a pressing need for in vivo models that more closely recapitulate human HSV infection. While the current in vivo, ex vivo, and in vitro models used to study HSV latency have limitations, they provide further insights that add to our understanding of latency. In vivo models have shed light on natural infection routes and the interplay between the host immune response and the virus during latency, while in vitro models have been invaluable in elucidating molecular pathways involved in latency. Below, we review the relative advantages and disadvantages of current HSV models and highlight insights gained through each.
摘要：
我们目前对HSV潜伏期的理解是基于各种临床观察，在体内，离体,和体外模型系统，每个都有独特的优点和缺点。对HSV潜伏期进行真实建模的标准包括易于操纵宿主遗传学和生物学途径的能力，以及模拟人类感染中的免疫反应和病毒发病机理。尽管在选择模型时需要对HSV延迟进行现实建模，成本,时间要求,道德约束，和试剂的可用性也同样重要。目前,仍然迫切需要更紧密地概括人HSV感染的体内模型。而体内的电流,离体,用于研究HSV潜伏期的体外模型有局限性，它们提供了进一步的见解，增加了我们对延迟的理解。体内模型揭示了自然感染途径以及潜伏期宿主免疫反应与病毒之间的相互作用。而体外模型在阐明与潜伏期有关的分子途径方面具有不可估量的价值。下面,我们回顾了当前HSV模型的相对优势和劣势，并重点介绍了通过每种模型获得的见解。
