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Abstract：
Background and Objectives: Connexin 43 (Cx43) is involved in the transfer of small signaling molecules between neighboring cells, thereby exerting a major influence on the initiation and progression of tumorigenesis. However, there is a lack of systematic research on Cx43 expression and its predictive role in clinical diagnosis and prognosis in pan-cancer. Materials and Methods: Several biological databases were used to evaluate the expression levels of GJA1 (encoding Cx43) and its diagnostic and prognostic significance in pan-cancer. We targeted kidney renal clear cell carcinoma (KIRC) and investigated the relationship between GJA1 expression and different clinical features of KIRC patients. Then, we performed cell-based experiments to partially confirm our results and predicted several proteins that were functionally related to Cx43. Results: The expression of GJA1 has a high level of accuracy in predicting KIRC. High GJA1 expression was remarkably correlated with a favorable prognosis, and this expression was reduced in groups with poor clinical features in KIRC. Cell experiments confirmed the inhibitory effects of increased GJA1 expression on the migratory capacity of human renal cancer (RCC) cell lines, and protein-protein interaction (PPI) analysis predicted that CDH1 and CTNNB1 were closely related to Cx43. Conclusions: GJA1 could be a promising independent favorable prognostic factor for KIRC, and upregulation of GJA1 expression could inhibit the migratory capacity of renal cancer cells.
摘要：
背景与目的:连接蛋白43(Cx43)参与小信号分子在邻近细胞间的传递,从而对肿瘤发生的发生和发展产生重大影响。然而,Cx43在泛癌症中的表达及其对临床诊断和预后的预测作用缺乏系统的研究。材料和方法：使用几个生物学数据库来评估GJA1（编码Cx43）的表达水平及其在泛癌症中的诊断和预后意义。我们以肾透明细胞癌（KIRC）为目标，研究GJA1表达与KIRC患者不同临床特征之间的关系。然后，我们进行了基于细胞的实验以部分证实我们的结果,并预测了与Cx43功能相关的几种蛋白质.结果：GJA1的表达在预测KIRC方面具有较高的准确性。高GJA1表达与良好预后显著相关,在KIRC临床特征较差的组中，该表达降低。细胞实验证实了GJA1表达增加对人肾癌（RCC）细胞系迁移能力的抑制作用，蛋白-蛋白相互作用(PPI)分析预测CDH1和CTNNB1与Cx43密切相关。结论：GJA1可能是KIRC的一个有前途的独立有利预后因素，GJA1表达上调可抑制肾癌细胞的迁移能力。
