PDF(Pubmed)
Abstract：
Previous studies on the early interference of gut microbiota by Bacillus siamensis (B. siamensis) in weaned piglets are rarely reported, and the present trial is a preliminary study. This experiment was conducted to investigate the effects of B. siamensis supplementation on the growth performance, serum biochemistry, immune response, fecal short-chain fatty acids and microbiota of weaned piglets. Sixty weaned piglets were randomly divided into a control group (CON) and a B. siamensis group (BS), which were fed a basal diet and the basal diet supplemented with 5 × 1010 CFU B. siamensis per kg, respectively. Each group had 3 replicates and 10 piglets per replicate. The trial lasted for 28 days. The results showed that B. siamensis significantly increased the serum growth hormone (GH) and insulin-like growth factor (IGF) in piglets. Compared with the CON group, the levels of serum immunoglobulin and inflammatory factors in the BS group were significantly improved. In addition, the serum concentrations of zonulin and endotoxin (ET) in the BS group were lower. The dietary addition of B. siamensis significantly increased fecal short-chain fatty acid (SCFA) levels in piglets. Notably, B. siamensis improved the microbial composition by increasing beneficial genera, including Weissella, Lachnospiraceae_NK4A136_group and Bifidobacterium, and decreasing pathogenic genera, including Pantoea, Fusobacterium and Gemella, in piglet feces. Correlation analysis showed that the benefits of dietary B. siamensis supplementation were closely related to its improved microbial composition. In summary, the addition of B. siamensis can improve the immunity function, inflammatory response, gut permeability and SCFA levels of weaned piglets, which may be achieved through the improvement of their microbiota.
摘要：
关于唾液酸芽孢杆菌早期干扰肠道菌群的研究（B.siamensis）在断奶仔猪中的报道很少，本试验为初步研究。本试验旨在研究饲粮中添加黄曲霉对其生长性能的影响,血清生物化学,免疫反应，断奶仔猪粪便短链脂肪酸和微生物群。将60头断奶仔猪随机分为对照组(CON)和赛马布组(BS),饲喂基础日粮和基础日粮，每公斤补充5×1010CFUB.siamensis，分别。每组有3个重复,每个重复10只小猪。审判持续了28天。结果表明,山葵能显著提高仔猪血清生长激素(GH)和胰岛素样生长因子(IGF)水平。与CON组相比,BS组血清免疫球蛋白和炎症因子水平明显改善。此外,BS组血清zonulin和内毒素（ET）浓度较低。饲粮中添加N.S.siamensis显著提高了仔猪粪便短链脂肪酸（SCFA）水平。值得注意的是,B.siamensis通过增加有益的属来改善微生物组成，包括Weissella,落叶松科_NK4A136_群和双歧杆菌，减少病原属，包括泛菌,梭杆菌和Gemella,在仔猪粪便中。相关性分析显示,日粮中添加赛马芽孢杆菌的益处与其改善的微生物组成密切相关。总之,添加B.siamensis可以提高免疫功能，炎症反应，断奶仔猪的肠道通透性和SCFA水平，这可以通过改善他们的微生物区系来实现。
