PDF(Pubmed)
Abstract：
Multiple sclerosis (MS) arises from a complex interplay between host genetic factors and environmental components, with the gut microbiota emerging as a key area of investigation. In the current study, we used ion torrent sequencing to delve into the bacteriome (bacterial microbiota) and mycobiome (fungal microbiota) of people with MS (pwMS), and compared them to healthy controls (HC). Through principal coordinate, diversity, and abundance analyses, as well as clustering and cross-kingdom microbial correlation assessments, we uncovered significant differences in the microbial profiles between pwMS and HC. Elevated levels of the fungus Torulaspora and the bacterial family Enterobacteriaceae were observed in pwMS, whereas beneficial bacterial taxa, such as Prevotelladaceae and Dialister, were reduced. Notably, clustering analysis revealed overlapping patterns in the bacteriome and mycobiome data for 74% of the participants, with weakened cross-kingdom interactions evident in the altered microbiota of pwMS. Our findings highlight the dysbiosis of both bacterial and fungal microbiota in MS, characterized by shifts in biodiversity and composition. Furthermore, the distinct disease-associated pattern of fungi-bacteria interactions suggests that fungi, in addition to bacteria, contribute to the pathogenesis of MS. Overall, our study sheds light on the intricate microbial dynamics underlying MS, paving the way for further investigation into the potential therapeutic targeting of the gut microbiota in MS management.
摘要：
多发性硬化症（MS）源于宿主遗传因素和环境成分之间复杂的相互作用，随着肠道微生物群成为一个关键的调查领域。在目前的研究中,我们使用离子洪流测序来深入研究MS（pwMS）患者的细菌组（细菌微生物群）和分枝杆菌组（真菌微生物群），并将其与健康对照（HC）进行比较。通过主要协调，多样性,和丰度分析，以及聚类和跨界微生物相关性评估，我们发现pwMS和HC之间的微生物谱存在显着差异。在pwMS中观察到真菌Torulaspora和细菌类肠杆菌科的水平升高，而有益的细菌分类群，如普雷沃拉达科和Dialister，减少了。值得注意的是,聚类分析显示，74%的参与者的细菌组和分枝杆菌组数据存在重叠模式，在pwMS改变的微生物群中明显具有减弱的跨王国相互作用。我们的发现强调了MS中细菌和真菌微生物群的菌群失调，以生物多样性和成分的变化为特征。此外，真菌-细菌相互作用的独特疾病相关模式表明真菌，除了细菌,有助于MS的发病机制。总的来说,我们的研究揭示了MS背后复杂的微生物动力学，为进一步研究MS管理中肠道微生物群的潜在治疗靶向奠定了基础。
