关键词: LAMP SaLux19 Sus scrofa (wild boar/pig) point-of-care testing species identification

来  源:   DOI:10.3390/life14050579   PDF(Pubmed)

Abstract:
Our research has developed a highly sensitive and simple assay to detect small amounts of animal and human biological material in less than 40 min. The handheld SaLux19 device developed at the Max Planck Institute of Experimental Medicine in Göttingen, Germany, was used to validate our concept. The proposed system uses isothermal amplification of DNA in a rapid assay format. Our results show that the assay can detect Sus scrofa nucleic acids with very high sensitivity and specificity. This detection system has potential for forensic scenarios.
摘要:
我们的研究开发了一种高灵敏度和简单的检测方法,可以在不到40分钟的时间内检测少量的动物和人类生物材料。在哥廷根的马克斯·普朗克实验医学研究所开发的手持式SaLux19设备,德国,被用来验证我们的概念。所提出的系统以快速测定形式使用DNA的等温扩增。我们的结果表明,该测定法可以以非常高的灵敏度和特异性检测Susscrofa核酸。该检测系统具有用于取证场景的潜力。
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