PDF(Pubmed)
Abstract：
Rana temporaria is one of the most widespread Palearctic brown frogs. We aimed to clarify distribution pattern of two main genetic clades in the understudied Balkan peninsula by using 16SrRNA and MT-CYTB sequences, already widely applied in analyses of populations from other parts of Europe, while focusing on the broad area along the Morava river (central Balkans) as a known gap in the species distribution. Additionally, we were interested in revealing the extent of haplotype diversity within the main genetic clades in the Balkans, particularly around the supposed suture zone. The results revealed a suture zone between the Western and Eastern Clades in the central part of the Balkan Peninsula. This indicated the existence of a historical barrier between the Balkan Mountain Belt and geographically close mountains surrounding the Vlasina Plateau (Rhodope/Serbian-Macedonian Massif). The overall observed haplotype diversity in populations of R. temporaria from the Balkan Peninsula seems high. Harboring both main genetic clades of R. temporaria qualifies the Balkan Peninsula as another important center of species\' genetic diversity, as well as rich in unique haplotypes. This points out the necessity of applying conservation measures focused on the common European frog populations and habitats in this part of the species\' distribution area.
摘要：
临时林蛙是最普遍的古北棕色青蛙之一。我们旨在通过使用16SrRNA和MT-CYTB序列来阐明巴尔干半岛中两个主要遗传进化枝的分布模式。已经广泛应用于欧洲其他地区的人口分析，同时将重点放在莫拉瓦河沿岸的广阔区域（巴尔干地区中部）作为物种分布的已知差距。此外,我们有兴趣揭示巴尔干地区主要遗传进化枝的单倍型多样性程度，特别是在假定的缝合区周围。结果显示，巴尔干半岛中部的西部和东部分支之间存在缝合区。这表明巴尔干山脉带与弗拉西纳高原（Rhodope/塞尔维亚-马其顿地块）周围地理上封闭的山脉之间存在历史障碍。巴尔干半岛的R.temporaria种群中观察到的整体单倍型多样性似乎很高。拥有R.temporaria的两个主要遗传分支使巴尔干半岛成为另一个重要的物种遗传多样性中心，以及丰富的独特单倍型。这指出了在物种分布区域的这一部分中，针对欧洲常见的青蛙种群和栖息地采取保护措施的必要性。
