PDF(Pubmed)
Abstract：
The yak is a unique species of livestock found in the Qinghai-Tibet Plateau and its surrounding areas. Due to factors such as late sexual maturity and a low rate of estrus, its reproductive efficiency is relatively low. The process of estrus synchronization in yaks plays a crucial role in enhancing their reproductive success and ensuring the continuation of their species. In order to clarify the characteristics of the serum metabolites of yak estrus synchronization, the yaks with inactive ovaries were compared with the estrus synchronization yaks. In this study, yaks were divided into the inactive ovaries group (IO), gonarelin-induced yak estrus group (GnRH), and chloprostenol sodium-induced yak estrus group (PGF). After the completion of the estrus synchronization treatment, blood samples were collected from the jugular veins of the non-estrus yaks in the control group and the yaks with obvious estrus characteristics in the GnRH and PGF groups. Metabolites were detected by ultra-high performance liquid chromatography-mass spectrometry, and differential metabolites were screened by multivariate statistical analysis. The results showed that a total of 70 significant differential metabolites were screened and identified in the GnRH vs. IO group, and 77 significant differential metabolites were screened and identified in the PGF vs. IO group. Compared with non-estrus yaks, 36 common significant differential metabolites were screened out after the induction of yak estrus by gonarelin (GnRH) and cloprostenol sodium (PGF), which were significantly enriched in signaling pathways such as the beta oxidation of very long chain fatty acids, bile acid biosynthesis, oxidation of branched chain fatty acids, steroidogenesis, steroid biosynthesis, and arginine and proline metabolism. This study analyzed the effects of gonadotropin releasing hormone (GnRH) and prostaglandin F (PGF) on the reproductive performance of yaks treated with estrus synchronization, which provides a theoretical basis for the optimization and application of yak estrus synchronization technology and promotes the healthy development of the yak industry.
摘要：
牦牛是在青藏高原及其周边地区发现的一种独特的牲畜。由于性成熟晚、发情率低等因素,其繁殖效率相对较低。牦牛的发情同步过程对提高其繁殖成功率和确保其物种的延续起着至关重要的作用。为了阐明同步发情牦牛血清代谢产物的特点,将卵巢不活跃的牦牛与发情同步牦牛进行比较。在这项研究中,牦牛被分为卵巢不活跃组(IO)，促性腺激素诱导牦牛发情期组(GnRH),和氯前列醇钠诱导牦牛发情组(PGF)。完成发情同步治疗后，从对照组的非发情牦牛的颈静脉和GnRH和PGF组中具有明显发情特征的牦牛的颈静脉采集血样。代谢物检测采用超高效液相色谱-质谱,并通过多变量统计分析筛选差异代谢产物。结果表明，在GnRH和IO组,并在PGF与PGF中筛选和鉴定了77种显着差异的代谢物。IO组。与非发情牦牛相比,用促性腺激素(GnRH)和氯前列醇钠(PGF)诱导牦牛发情后,筛选出36种常见显著差异代谢产物,它们在信号通路中显著富集，如非常长链脂肪酸的β氧化，胆汁酸生物合成，支链脂肪酸的氧化，类固醇生成，类固醇生物合成，精氨酸和脯氨酸代谢。本研究分析了促性腺激素释放激素(GnRH)和前列腺素F(PGF)对同步发情牦牛繁殖性能的影响,为牦牛发情同步技术的优化与应用提供了理论依据,促进了牦牛产业的健康发展。
