PDF(Pubmed)
Abstract：
Chronic venous disease (CVD) comprises a spectrum of morphofunctional disorders affecting the venous system, affecting approximately 1 in 3 women during gestation. Emerging evidence highlights diverse maternofetal implications stemming from CVD, particularly impacting the placenta. While systemic inflammation has been associated with pregnancy-related CVD, preliminary findings suggest a potential link between this condition and exacerbated inflammation in the placental tissue. Inflammasomes are major orchestrators of immune responses and inflammation in different organs and systems. Notwithstanding the relevance of inflammasomes, specifically the NLRP3 (nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3)- which has been demonstrated in the placentas of women with different obstetric complications, the precise involvement of this component in the placentas of women with CVD remains to be explored. This study employs immunohistochemistry and real-time PCR (RT-qPCR) to examine the gene and protein expression of key components in both canonical and non-canonical pathways of the NLRP3 inflammasome (NLRP3, ASC-apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain-caspase 1, caspase 5, caspase 8, and interleukin 1β) within the placental tissue of women affected by CVD. Our findings reveal a substantial upregulation of these components in CVD-affected placentas, indicating a potential pathophysiological role of the NLRP3 inflammasome in the development of this condition. Subsequent investigations should focus on assessing translational interventions addressing this dysregulation in affected patient populations.
摘要：
慢性静脉疾病（CVD）包括一系列影响静脉系统的形态功能障碍，在妊娠期间影响大约三分之一的女性。新出现的证据强调了源于CVD的不同母胎含义，特别是影响胎盘。虽然全身性炎症与妊娠相关的CVD有关，初步研究结果表明，这种情况与胎盘组织炎症加剧之间存在潜在联系。炎症小体是不同器官和系统中免疫反应和炎症的主要协调者。尽管炎症相关，特别是NLRP3(核苷酸结合域，富含亮氨酸的家族，含pyrin结构域-3）-已在患有不同产科并发症的女性的胎盘中得到证实，这一部分在心血管疾病女性胎盘中的确切参与还有待探讨.本研究采用免疫组织化学和实时PCR（RT-qPCR）来检查NLRP3炎性体（NLRP3，ASC-凋亡相关斑点样蛋白）的规范和非规范途径中关键成分的基因和蛋白质表达。受CVD影响的女性组织内的caspaseplac5，caspase8和白介素1β。我们的发现揭示了在受CVD影响的胎盘中这些成分的显著上调，表明NLRP3炎性体在这种情况的发展中的潜在病理生理作用。随后的调查应侧重于评估在受影响的患者人群中解决这种失调的转化干预措施。
