PDF(Pubmed)
Abstract：
Rose roxburghii, a horticulturally significant species within the Rosa genus of the Rosaceae family, is renowned for its abundance of secondary metabolites and ascorbate, earning it the title \'king of vitamin C\'. Despite this recognition, the mechanisms underlying the biosynthesis and regulation of triterpenoid compounds in R. roxburghii remain largely unresolved. In this study, we conducted high-performance liquid chromatography profiling across various organs of R. roxburghii, including fruit, root, stem, and leaves, revealing distinct distributions of triterpenoid compounds among different plant parts. Notably, the fruit exhibited the highest total triterpenoid content, followed by root and stem, with leaf containing the lowest levels, with leaf containing the lowest levels. Transcriptomic analysis unveiled preferential expression of members from the cytochrome P450 (CYP) and glycosyltransferase (UGT) families, likely contributing to the higher accumulation of both ascorbate and triterpenoid compounds in the fruits of R. roxburghii compared to other tissues of R. roxburghii. Transcriptomic analysis unveiled a potential gene network implicated in the biosynthesis of both ascorbate and triterpenoid compounds in R. roxburghii. These findings not only deepen our understanding of the metabolic pathways in this species but also have implications for the design of functional foods enriched with ascorbate and triterpenoids in R. roxburghii.
摘要：
玫瑰刺梨,玫瑰科玫瑰属中的园艺重要物种，以其丰富的次级代谢产物和抗坏血酸盐而闻名，赢得了“维生素C之王”的称号。尽管有这样的认可,刺梨三萜类化合物的生物合成和调节机制在很大程度上仍未解决。在这项研究中,我们对刺梨的各个器官进行了高效液相色谱分析，包括水果,根,茎,和叶子，揭示了三萜化合物在不同植物部位的不同分布。值得注意的是,果实表现出最高的总三萜含量，其次是根和茎，叶子含有最低水平，叶子含有最低水平。转录组学分析揭示了细胞色素P450（CYP）和糖基转移酶（UGT）家族成员的优先表达，与刺梨的其他组织相比，刺梨果实中抗坏血酸和三萜化合物的积累可能更高。转录组学分析揭示了与刺梨中抗坏血酸和三萜化合物的生物合成有关的潜在基因网络。这些发现不仅加深了我们对该物种代谢途径的理解，而且对刺梨中富含抗坏血酸和三萜类化合物的功能性食品的设计也有意义。
